Mouse APOE ELISA Kit
Sensitivity
0.05 ng/mL
Range
0.156-10 ng/mL
Reactivity
Mouse
Validation Data Gallery
Product Information
KE10131 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse APOE ELISA kit is to be used to detect and quantify protein levels of endogenous mouse APOE. The assay recognizes mouse APOE. An antibody specific for mouse APOE has been pre-coated onto the microwells. The mouse APOE protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse APOE is added to detect the captured mouse APOE protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse APOE ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.05 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 11816 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 100% | 94%-120% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 6.14 | 0.30 | 4.90 |
| 2 | 20 | 1.12 | 0.03 | 2.78 |
| 3 | 20 | 0.38 | 0.02 | 4.52 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 6.66 | 0.59 | 8.93 |
| 2 | 24 | 1.14 | 0.03 | 2.47 |
| 3 | 24 | 0.41 | 0.02 | 3.73 |
Background Information
Apolipoprotein E (APOE) is a core component of plasma lipoproteins and is involved in a variety of biological processes, including plasma lipoprotein metabolism, intracellular cholesterol utilization, cell growth, immune regulation, and neuronal growth and repair.The ε4 allele of the APOE gene is the strongest genetic risk factor for late-onset Alzheimer's disease (AD). Mouse APOE, like ApoE4, contains equivalents of Arg-112 and Glu-255 but lacks the critical Arg-61 equivalent. APOE has heparin-binding activity and binds to heparan sulfate proteoglycans on the cell surface, a property that facilitates cellular capture and receptor-mediated uptake of APOE-containing lipoproteins.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Apoe,AI255918,apolipoprotein E |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
