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验证数据展示

  • WB analysis using 29133-1-AP

    WB analysis using 29133-1-AP

    Untreated and Trichostatin A treated HeLa cell lysates were subjected to SDS PAGE followed by western blot with 29133-1-AP (Mono-Acetyl-Histone H3 (Lys9) antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 29133-1-AP

    WB analysis of HEK-293 using 29133-1-AP

    WB result of Mono-Acetyl-Histone H3 (Lys9) antibody (29133-1-AP; 1:2400; incubated at room temperature for 1.5 hours) with sh-Control and sh-Mono-Acetyl-Histone H3 (Lys9) transfected HEK-293 cells.

  • Featured Product
  • KD/KO验证

Acetyl-Histone H3 (Lys9) Polyclonal antibody

Acetyl-Histone H3 (Lys9) Polyclonal Antibody for WB, ELISA
Cat No. 29133-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, ELISA and More (1)

别名

HIST1H3A, Histone-H3, HistoneH3, Histone H3, Histone H 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy Phospho-Histone H3 (Ser10) Ready-To-Use IHC Kit. Phospho-Histone H3 (Ser10) primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29133-1-AP targets Acetyl-Histone H3 (Lys9) in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 histone cluster 1, H3a
别名 HIST1H3A, Histone-H3, HistoneH3, Histone H3, Histone H 3
观测分子量15 kDa
GenBank蛋白编号BC066245
基因名称 HIST1H3A
Gene ID (NCBI) 8350
RRIDAB_3085344
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP68431
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified.

实验方案

Product Specific Protocols
WB protocol for Acetyl-Histone H3 (Lys9) antibody 29133-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratIHC

Neurochem Res

The Role of Acetylation and Methylation of Rat Hippocampal Histone H3 in the Mechanism of Aluminum-Induced Neurotoxicity

Authors - Jie Gao
View Article
humanWB

Nature

Transcriptional adaptation upregulates utrophin in Duchenne muscular dystrophy

Authors - Lara Falcucci
View Article