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验证数据展示

  • WB analysis using 33084-1-AP

    WB analysis using 33084-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 33084-1-AP (Mmp8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse spleen using 33084-1-AP

    IF Staining of mouse spleen using 33084-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using Mmp8 antibody (33084-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Mmp8 Polyclonal antibody

Mmp8 Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 33084-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IF-P, ELISA

别名

Collagenase 2, EC:3.4.24.34, Matrix metalloproteinase-8, MMP-8, Neutrophil collagenase

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse lung tissue, mouse spleen tissue, rat lung tissue, rat spleen tissue
Positive IF-P detected inmouse spleen tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

33084-1-AP targets Mmp8 in WB, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 mouse, rat
免疫原

CatNo: Eg3161

Product name: recombinant mouse Mmp8 protein

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 21-465 aa of NM_008611.4

Sequence: FPVPEHLEEKNIKTAENYLRKFYNLPSNQFRSSRNATMVAEKLKEMQRFFSLAETGKLDAATMGIMEMPRCGVPDSGDFLLTPGSPKWTHTNLTYRIINHTPQLSRAEVKTAIEKAFHVWSVASPLTFTEILQGEADINIAFVSRDHGDNSPFDGPNGILAHAFQPGQGIGGDAHFDSEETWTQDSKNYNLFLVAAHEFGHSLGLSHSTDPGALMYPNYAYREPSTYSLPQDDINGIQTIYGPSDNPIQPTGPSTPKACDPHLRFDATTTLRGEIYFFKDKYFWRRHPQLRTVDLNFISLFWPFLPNGLQAAYEDFDRDLVFLFKGRQYWALSGYDLQQGYPRDISNYGFPRSVQAIDAAVSYNGKTYFFINNQCWRYDNQRRSMDPGYPKSIPSMFPGVNCRVDAVFLQDSFFLFFSGPQYFAFNFVSHRVTRVARSNLWLNCS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 matrix metallopeptidase 8
别名 Collagenase 2, EC:3.4.24.34, Matrix metalloproteinase-8, MMP-8, Neutrophil collagenase
计算分子量53 kDa
观测分子量 60-70 kDa
GenBank蛋白编号NM_008611.4
基因名称 Mmp8
Gene ID (NCBI) 17394
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDO70138
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MMP8(Matrix metalloproteinase-8) an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells(PMID:15367487). Purified human neutrophil MMP-8 with proeMMP-8 at 70-75 KDa and active enzyme at 65 KDa and amniochorion proenzyme and active enzyme at 65 and 50 KDa, respectively(PMID: 28772283, PMID:15042023).

实验方案

Product Specific Protocols
IF protocol for Mmp8 antibody 33084-1-APDownload protocol
WB protocol for Mmp8 antibody 33084-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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