×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis of SK-MEL-30 using 87351-4-RR (same clone as 87351-4-PBS)

    WB analysis of SK-MEL-30 using 87351-4-RR (same clone as 87351-4-PBS)

    SK-MEL-30 cells were subjected to SDS PAGE followed by western blot with 87351-4-RR (MLANA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87351-4-PBS in a different storage buffer formulation.

  • IHC staining of human malignant melanoma using 87351-4-PBS

    IHC staining of human malignant melanoma using 87351-4-PBS

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 87351-4-PBS (Melan-A antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Melan-A antibody included
  • IHC staining of human skin using 87351-4-RR (same clone as 87351-4-PBS)

    IHC staining of human skin using 87351-4-RR (same clone as 87351-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 87351-4-RR (MLANA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87351-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Melan-A antibody included
  • IF Staining of SK-MEL-28 using 87351-4-RR (same clone as 87351-4-PBS)

    IF Staining of SK-MEL-28 using 87351-4-RR (same clone as 87351-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SK-MEL-28 cells using Melan-A antibody (87351-4-RR, Clone: 252647C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 87351-4-PBS in a different storage buffer formulation.

Melan-A Recombinant monoclonal antibody, PBS Only

Melan-A Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 87351-4-PBS

产品说明书

CloneNo. 252647C9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

melan A, MLANA, Antigen LB39 AA, Antigen LB39-AA, Antigen SK29 AA

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IHC detected inhuman malignant melanoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy Melan-A Ready-To-Use IHC Kit. Melan-A primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87351-4-PBS targets Melan-A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag13346

Product name: Recombinant human MLANA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-118 aa of BC014423

Sequence: MPREDAHFIYGYPKKGHGHSYTTAEEAAGIGILTVILGVLLLIGCWYCRRRNGYRALMDKSLHVGTQCALTRRCPQEGFDHRDSKVSLQEKNCEPVVPNAPPAYEKLSAEQSPPPYSP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 melan-A
别名 melan A, MLANA, Antigen LB39 AA, Antigen LB39-AA, Antigen SK29 AA
计算分子量 13 kDa
观测分子量 13-20 kDa
GenBank蛋白编号BC014423
基因名称 MelanA
Gene ID (NCBI) 2315
RRIDAB_3745484
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ16655
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Melan-A is a melanocyte differentiation antigen, recognized by autologous cytotoxic T lymphocytes. Melan-A is also called MART-1 (melanoma antigen recognized by T cells). The Melan-A/MART-1 gene encodes this protein, 20-22 kDa, associated with endoplasmic reticulum and melanosomes. The function of the protein is unknown. Melan-A, isolated as a melanoma-specific antigen, is a transmembrane protein, which is expressed in skin, retina and the majority of cultured melanocytes as well as in melanomas. Melan A is expressed in more than 85% of melanomas.

||
New chat

Able™

正在加载，请稍候...