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验证数据展示

  • WB analysis of SK-MEL-30 using 87351-4-RR (same clone as 87351-4-PBS)

    WB analysis of SK-MEL-30 using 87351-4-RR (same clone as 87351-4-PBS)

    SK-MEL-30 cells were subjected to SDS PAGE followed by western blot with 87351-4-RR (MLANA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87351-4-PBS in a different storage buffer formulation.

  • IHC staining of human skin using 87351-4-RR (same clone as 87351-4-PBS)

    IHC staining of human skin using 87351-4-RR (same clone as 87351-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 87351-4-RR (MLANA antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87351-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Melan-A antibody included

Melan-A Recombinant monoclonal antibody, PBS Only

Melan-A Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 87351-4-PBS

产品说明书

CloneNo. 252647C9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

melan A, MLANA, Antigen LB39 AA, Antigen LB39-AA, Antigen SK29 AA

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy Melan-A Ready-To-Use IHC Kit. Melan-A primary antibody included.

产品信息

87351-4-PBS targets Melan-A in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag13346

Product name: Recombinant human MLANA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-118 aa of BC014423

Sequence: MPREDAHFIYGYPKKGHGHSYTTAEEAAGIGILTVILGVLLLIGCWYCRRRNGYRALMDKSLHVGTQCALTRRCPQEGFDHRDSKVSLQEKNCEPVVPNAPPAYEKLSAEQSPPPYSP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 melan-A
别名 melan A, MLANA, Antigen LB39 AA, Antigen LB39-AA, Antigen SK29 AA
计算分子量 13 kDa
观测分子量 13-20 kDa
GenBank蛋白编号BC014423
基因名称 MelanA
Gene ID (NCBI) 2315
RRIDAB_3745484
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ16655
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Melan-A is a melanocyte differentiation antigen, recognized by autologous cytotoxic T lymphocytes. Melan-A is also called MART-1 (melanoma antigen recognized by T cells). The Melan-A/MART-1 gene encodes this protein, 20-22 kDa, associated with endoplasmic reticulum and melanosomes. The function of the protein is unknown. Melan-A, isolated as a melanoma-specific antigen, is a transmembrane protein, which is expressed in skin, retina and the majority of cultured melanocytes as well as in melanomas. Melan A is expressed in more than 85% of melanomas.

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