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  • Featured Product
  • KD/KO Validated

Mecr Polyclonal antibody

Mecr Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 51025-2-Ig

Print datasheet

Synonyms

AI195831, Mecr, Nrbf1

验证数据展示

  • WB analysis of mouse skeletal muscle using 51025-2-Ig

    WB analysis of mouse skeletal muscle using 51025-2-Ig

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 51025-2-Ig (Mecr antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 51025-2-Ig

    IHC staining of mouse skeletal muscle using 51025-2-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 51025-2-Ig (Mecr antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, mouse heart tissue
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

51025-2-Ig targets Mecr in WB, IHC, ELISA applications and shows reactivity with mouse samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity mouse
Cited Reactivitymouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitochondrial trans-2-enoyl-CoA reductase
Synonyms AI195831, Mecr, Nrbf1
Calculated Molecular Weight 40 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC003864
Gene Symbol Mecr
Gene ID (NCBI) 26922
RRIDAB_1167469
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9DCS3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Mecr antibody 51025-2-IgDownload protocol
IHC protocol for Mecr antibody 51025-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Redox Biol

Non-electron transfer chain mitochondrial defects differently regulate HIF-1α degradation and transcription.

Authors - Antonina N Shvetsova
  • KD Validated
View Article
mouseWB

Biochem Biophys Res Commun

The mitochondrial fatty acid synthesis (mtFASII) pathway is capable of mediating nuclear-mitochondrial cross talk through the PPAR system of transcriptional activation.

Authors - Parl Angelika A
View Article