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验证数据展示

  • WB analysis of HepG2 using 11722-1-AP (same clone as 11722-1-PBS)

    WB analysis of HepG2 using 11722-1-AP (same clone as 11722-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11722-1-AP (Maspin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11722-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 11722-1-AP (same clone as 11722-1-PBS)

    WB analysis of HeLa using 11722-1-AP (same clone as 11722-1-PBS)

    WB result of Maspin antibody (11722-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Maspin transfected HeLa cells. This data was developed using the same antibody clone with 11722-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 11722-1-AP (same clone as 11722-1-PBS)

    IHC staining of human lung cancer using 11722-1-AP (same clone as 11722-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11722-1-AP (Maspin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11722-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 11722-1-AP (same clone as 11722-1-PBS)

    IF Staining of A431 using 11722-1-AP (same clone as 11722-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using Maspin antibody (11722-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11722-1-PBS in a different storage buffer formulation.

  • IHC staining of human bowen disease using 11722-1-AP (same clone as 11722-1-PBS)

    IHC staining of human bowen disease using 11722-1-AP (same clone as 11722-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 11722-1-AP (Maspin antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11722-1-PBS in a different storage buffer formulation.

  • IHC staining of human bowen disease using 11722-1-AP (same clone as 11722-1-PBS)

    IHC staining of human bowen disease using 11722-1-AP (same clone as 11722-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 11722-1-AP (Maspin antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11722-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Maspin Polyclonal antibody, PBS Only

Maspin Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 11722-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

SERPINB5, Peptidase inhibitor 5, PI 5, PI5, PI-5

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

11722-1-PBS targets Maspin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag2304

Product name: Recombinant human SERPINB5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-231 aa of BC020713

Sequence: MDALQLANSAFAVDLFKQLCEKEPLGNVLFSPICLSTSLSLAQVGAKGDTANEIGQVLHFENVKDVPFGFQTVTSDVNKLSSFYSLKLIKRLYVDKSLNLSTEFISSTKRPYAKELETVDFKDKLEETKGQINNSIKDLTDGHFENILADNSVNDQTKILVVNAAYFVGKWMKKFPESETKECPFRVNKVCGAACSSKRSPIIDVKNDRDRVGHKSIPMRNLRARPAKCLS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 serpin peptidase inhibitor, clade B (ovalbumin), member 5
别名 SERPINB5, Peptidase inhibitor 5, PI 5, PI5, PI-5
计算分子量 375 aa, 42 kDa
观测分子量 38-42 kDa
GenBank蛋白编号BC020713
基因名称 Maspin
Gene ID (NCBI) 5268
RRIDAB_10597111
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP36952
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SERPINB5, also named PI-5, Maspin, and Serpin B5, belongs to the serpin family and Ov-serpin subfamily. It is a tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, SERPINB5 exhibits no serine protease inhibitory activity.

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