验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HeLa cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25234-1-AP targets MYT1L in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag18698 Product name: Recombinant human MYT1L protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 160-473 aa of BC137273 Sequence: EEEEEEEENEDHQMNCHNTRIMQDTEKDDNNNDEYDNYDELVAKSLLNLGKIAEDAAYRARTESEMNSNTSNSLEDDSDKNENLGRKSELSLDLDSDVVRETVDSLKLLAQGHGVVLSENMNDRNYADSMSQQDSRNMNYVMLGKPMNNGLMEKMVEESDEEVCLSSLECLRNQCFDLARKLSETNPQERNPQQNMNIRQHVRPEEDFPGRTPDRNYSDMLNLMRLEEQLSPRSRVFASCAKEDGCHERDDDTTSVNSDRSEEVFDMTKGNLTLLEKAIALETERAKAMREKMAMEAGRRDNMRSYEDQSPRQL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | myelin transcription factor 1-like |
| 别名 | NZF1, MyT1-L, MyT1 L, Myelin transcription factor 1-like protein, KIAA1106 |
| 计算分子量 | 1186 aa, 133 kDa |
| 观测分子量 | 110 kDa |
| GenBank蛋白编号 | BC137273 |
| 基因名称 | MYT1L |
| Gene ID (NCBI) | 23040 |
| RRID | AB_2879978 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UL68 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MYT1L antibody 25234-1-AP | Download protocol |
| IHC protocol for MYT1L antibody 25234-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Neuron A MYT1L syndrome mouse model recapitulates patient phenotypes and reveals altered brain development due to disrupted neuronal maturation. | ||
Cell Prolif Direct reprogramming of fibroblasts into spiral ganglion neurons by defined transcription factors | ||
Brain Res Bull miRNA-338-3p inhibits glioma cell proliferation and progression by targeting MYT1L
| ||
J Biol Chem Neuronal splicing of the unmethylated histone H3K4 reader, PHF21A, prevents excessive synaptogenesis | ||
Nat Neurosci Age-related Huntington's disease progression modeled in directly reprogrammed patient-derived striatal neurons highlights impaired autophagy | ||
Genome Res MYT1L is required for suppressing earlier neuronal development programs in the adult mouse brain |
