验证数据展示

WB analysis of HeLa using 13751-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13751-1-AP (MYST2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 13751-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13751-1-AP (MYST2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 13751-1-AP
IP result of anti-MYST2 (IP:13751-1-AP, 4ug; Detection:13751-1-AP 1:500) with HeLa cells lysate 2480ug.
IF Staining of NIH/3T3 using 13751-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using 13751-1-AP (MYST2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC staining of human pancreas cancer using 13751-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13751-1-AP (MYST2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with KAT7/MYST2 antibody included
IHC staining of human pancreas cancer using 13751-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13751-1-AP (MYST2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with KAT7/MYST2 antibody included
IHC staining of human thyroid cancer using 13751-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 13751-1-AP (MYST2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with KAT7/MYST2 antibody included
IHC staining of human thyroid cancer using 13751-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 13751-1-AP (MYST2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with KAT7/MYST2 antibody included
IHC staining of mouse stomach using 13751-1-AP
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 13751-1-AP (MYST2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with KAT7/MYST2 antibody included
IHC staining of mouse stomach using 13751-1-AP
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 13751-1-AP (MYST2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with KAT7/MYST2 antibody included
IHC staining of rat stomach using 13751-1-AP
Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 13751-1-AP (MYST2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with KAT7/MYST2 antibody included
IHC staining of rat stomach using 13751-1-AP
Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 13751-1-AP (MYST2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with KAT7/MYST2 antibody included
IHC staining of rat testis using 13751-1-AP
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 13751-1-AP (MYST2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with KAT7/MYST2 antibody included
IHC staining of rat testis using 13751-1-AP
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 13751-1-AP (MYST2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with KAT7/MYST2 antibody included
IHC staining of human pancreas cancer using 13751-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13751-1-AP (MYST2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with KAT7/MYST2 antibody included

Featured Product
KD/KO验证

MYST2 Polyclonal antibody

MYST2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13751-1-AP

产品说明书

引用文献(20)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (3)

别名

KAT7, KAT 7, Histone acetyltransferase KAT7, HBOa, HBO1

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

偶联物: Unconjugated

Unconjugated

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (20)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	HeLa cells, human testis tissue, Jurkat cells, NIH/3T3 cells, MCF-7 cells, PC-3 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human pancreas cancer tissue, human thyroid cancer tissue, mouse stomach tissue, rat stomach tissue, rat testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	NIH/3T3 cells

Planning an IHC experiment? We recommend our IHCeasy KAT7/MYST2 Ready-To-Use IHC Kit. KAT7/MYST2 primary antibody included.

应用	推荐稀释比
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 2 publications below
WB	See 20 publications below
IHC	See 3 publications below
IF	See 4 publications below
IP	See 3 publications below
CoIP	See 1 publications below
ChIP	See 1 publications below

产品信息

13751-1-AP targets MYST2 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用	WB, IHC, IF, IP, CoIP, ChIP
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat
免疫原	CatNo: Ag4693 Product name: Recombinant human MYST2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-370 aa of BC032640 Sequence: MPRRKRNAGSSSDGTEDSDFSTDLEHTDSSESDGTSRRSARVTRSSARLSQSSQDSSPVRNLQSFGTEEPAYSTRRVTRSQQQPTPVTPKKYPLRQTRSSGSETEQVVDFSDRETKNTADHDESPPRTPTGNAPSSESDIDISSPNVSHDESIAKDMSLKDSGSDLSHRPKRRRFHESYNFNMKCPTPGCNSLGHLTGKHERHFSISGCPLYHNLSADECKVRAQSRDKQIEERMLSHRQDDNNRHATRHQAPTERQLRYKEKVAELRKKRNSGLSKEQKEKYMEHRQTYGNTREPLLENLTSEYDLDLFRRAQARASEDLEKLRLQGQITEGSNMIKTIAFGRYELDTWYHSPYPEEYARLGRLYMCEF 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	MYST histone acetyltransferase 2
别名	KAT7, KAT 7, Histone acetyltransferase KAT7, HBOa, HBO1
计算分子量	611 aa, 71 kDa
观测分子量	71 kDa
GenBank蛋白编号	BC032640
基因名称	MYST2
Gene ID (NCBI)	11143
RRID	AB_2266703
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	O95251
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

MYST2, also named as HBO1, HBOa MOZ, YBF2/SAS3, SAS2 and TIP60 protein 2, belongs to the MYST (SAS/MOZ) family. It specifically represses AR mediated transcription. MYST2 is a candidate oncogene. It enhances the anchorage-independent growth of breast cancer cells.(PMID:19372580) MYST2 is a histone acetyltransferase (HAT) which could exert oncogenic function in breast cancer. It is an important downstream molecule of ERa, and ERK1/2 signaling pathway may involved in the expression of HBO1 increased by E2.

实验方案

Product Specific Protocols
IF protocol for MYST2 antibody 13751-1-AP	Download protocol
IHC protocol for MYST2 antibody 13751-1-AP	Download protocol
IP protocol for MYST2 antibody 13751-1-AP	Download protocol
WB protocol for MYST2 antibody 13751-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB	Cancer Cell Transcriptional Regulation of the Warburg Effect in Cancer by SIX1. Authors - Ling Li KO Validated View Article
human	WB	Adv Sci (Weinh) Transcriptional Regulation of De Novo Lipogenesis by SIX1 in Liver Cancer Cells Authors - Ling Li View Article
human	WB,IF,IP	Autophagy KAT7-mediated CANX (calnexin) crotonylation regulates leucine-stimulated MTORC1 activity. Authors - Guokai Yan View Article
human	WB	Cancer Res Hbo1 is a cyclin E/CDK2 substrate that enriches breast cancer stem-like cells. Authors - Mylinh T Duong View Article
human	WB	Mol Cell Proteomics Acetylproteomic analysis reveals functional implications of lysine acetylation in human spermatozoa (sperm). Authors - Heguo Yu View Article
human	WB	Mol Cell Proteomics Regulation of acetylation restores proteolytic function of diseased myocardium in mouse and human. Authors - Ding Wang View Article

验证数据展示

WB analysis of HeLa using 13751-1-AP

WB analysis of Jurkat using 13751-1-AP

IP experiment of HeLa using 13751-1-AP

IF Staining of NIH/3T3 using 13751-1-AP

IHC staining of human pancreas cancer using 13751-1-AP

IHC staining of human pancreas cancer using 13751-1-AP

IHC staining of human thyroid cancer using 13751-1-AP

IHC staining of human thyroid cancer using 13751-1-AP

IHC staining of mouse stomach using 13751-1-AP

IHC staining of mouse stomach using 13751-1-AP

IHC staining of rat stomach using 13751-1-AP

IHC staining of rat stomach using 13751-1-AP

IHC staining of rat testis using 13751-1-AP

IHC staining of rat testis using 13751-1-AP

IHC staining of human pancreas cancer using 13751-1-AP

MYST2 Polyclonal antibody

MYST2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.

Transcriptional Regulation of De Novo Lipogenesis by SIX1 in Liver Cancer Cells

KAT7-mediated CANX (calnexin) crotonylation regulates leucine-stimulated MTORC1 activity.

Hbo1 is a cyclin E/CDK2 substrate that enriches breast cancer stem-like cells.

Acetylproteomic analysis reveals functional implications of lysine acetylation in human spermatozoa (sperm).

Regulation of acetylation restores proteolytic function of diseased myocardium in mouse and human.