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验证数据展示

  • WB analysis of rat skeletal muscle using 23880-1-AP

    WB analysis of rat skeletal muscle using 23880-1-AP

    rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 23880-1-AP (MYOZ3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skeletal muscle using 23880-1-AP

    IHC staining of human skeletal muscle using 23880-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 23880-1-AP (MYOZ3 Antibody) at dilution of 1:50 (under 40x lens).

MYOZ3 Polyclonal antibody

MYOZ3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 23880-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Calsarcin 3, CS 3, CS3, FATZ related protein 3, FRP3, MYOZ3, myozenin 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inrat skeletal muscle tissue, mouse skeletal muscle tissue
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23880-1-AP targets MYOZ3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag20839

Product name: Recombinant human MYOZ3 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-251 aa of BC074870

Sequence: MIPKEQKGPVMAAMGDLTEPVPTLDLGKKLSVPQDLMMEELSLRNNRGSLLFQKRQRRVQKFTFELAASQRAMLAGSARRKVTGTAESGTVANANGPEGPNYRSELHIFPASPGASLGGPEGAHPAAAPAGCVPSPSALAPGYAEPLKGVPPEKFNHTAIPKGYRCPWQEFVSYRDYQSDGRSHTPSPNDYRNFNKTPVPFGGPLVGGTFPRPGTPFIPEPLSGLELLRLRPSFNRVAQGWVRNLPESEEL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myozenin 3
别名 Calsarcin 3, CS 3, CS3, FATZ related protein 3, FRP3, MYOZ3, myozenin 3
计算分子量 251 aa, 27 kDa
观测分子量 33 kDa
GenBank蛋白编号BC074870
基因名称 MYOZ3
Gene ID (NCBI) 91977
RRIDAB_2879347
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ8TDC0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MYOZ3 antibody 23880-1-APDownload protocol
IHC protocol for MYOZ3 antibody 23880-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols