×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis of mouse skeletal muscle using 10731-1-AP (same clone as 10731-1-PBS)

    WB analysis of mouse skeletal muscle using 10731-1-AP (same clone as 10731-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10731-1-AP (MYOT antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10731-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 10731-1-AP (same clone as 10731-1-PBS)

    IHC staining of mouse skeletal muscle using 10731-1-AP (same clone as 10731-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 10731-1-AP (MYOT antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10731-1-PBS in a different storage buffer formulation.

MYOT Polyclonal antibody, PBS Only

MYOT Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 10731-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

产品信息

10731-1-PBS targets MYOT in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1112

Product name: Recombinant human MYOT protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 191-498 aa of BC005376

Sequence: GPQNAAAVFQAQDDSGAQDSQQHNSEHARLQVPTSQVRSRSTSRGDVNDQDAIQEKFYPPRFIQVPENMSIDEGRFCRMDFKVSGLPAPDVSWYLNGRTVQSDDLHKMIVSEKGLHSLIFEVVRASDAGAYACVAKNRAGEATFTVQLDVLAKEHKRAPMFIYKPQSKKVLEGDSVKLECQISAIPPPKLFWKRNNEMVQFNTDRISLYQDNTGRVTLLIKDVNKKDAGWYTVSAVNEAGVTTCNTRLDVTARPNQTLPAPKQLRVRPTFSKYLALNGKGLNVKQAFNPEGEFQRLAAQSGLYESEEL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myotilin
别名
计算分子量 55 kDa
观测分子量 55-57 kDa, 35 kDa
GenBank蛋白编号BC005376
基因名称 MYOT
Gene ID (NCBI) 9499
RRIDAB_2297956
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UBF9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MYOT (myotilin) is a structural protein of the striated muscle Z-discs. It interacts with both actinin and filamin, forming a complex to maintain structural stability of muscles. In adult tissues, myotilin is mainly expressed in skeletal and cardiac muscles and in the peripheral nerves. Missense mutations of myotilin cause limb girdle muscular dystrophy 1A and some other myopathy. Two isoforms of MYOT exist due to the alternative splicing. This antibody can detect both of isoforms around 57 kDa and 35 kDa.

||
New chat

Able™

正在加载，请稍候...