验证数据展示
经过测试的应用
| Positive WB detected in | mouse skeletal muscle tissue, rat skeletal muscle tissue |
| Positive IP detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | human skeletal muscle tissue, mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse skeletal muscle tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:400 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17692-1-AP targets MYOM3 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP, ELISA |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, canine |
| 免疫原 | MYOM3 fusion protein Ag11849 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | myomesin family, member 3 |
| 别名 | Myomesin-3, Myomesin family member 3, Myomesin 3 |
| 计算分子量 | 162 kDa, 136 kDa |
| 观测分子量 | 162 kDa |
| GenBank蛋白编号 | BC067101 |
| 基因名称 | MYOM3 |
| Gene ID (NCBI) | 127294 |
| RRID | AB_2146624 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5VTT5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MYOM3 (myomesin 3) is a structural component of the M-band in striated muscle and is involved in sarcomere stability and resistance during intense or sustained stretching. MYOM3 can be detected mainly in intermediate-speed fibers of skeletal muscle. Recently high levels of MYOM3 fragments were detected in sera from patients with muscular dystrophy, including Duchenne muscular dystrophy (DMD). MYOM3 fragments may be used as serum biomarkers for DMD and other neuromuscular disorders. This antibody recognizes the intact MYOM3 protein (160-162 kDa) as well as MYOM3 fragments (100 kDa and 130 kDa). (26060189)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MYOM3 antibody 17692-1-AP | Download protocol |
| IHC protocol for MYOM3 antibody 17692-1-AP | Download protocol |
| IF protocol for MYOM3 antibody 17692-1-AP | Download protocol |
| IP protocol for MYOM3 antibody 17692-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun An engineered AAV targeting integrin alpha V beta 6 presents improved myotropism across species | ||
J Clin Invest A functional mini-GDE transgene corrects impairment in models of glycogen storage disease type III | ||
Nucleic Acids Res Palmitic acid conjugation enhances potency of tricyclo-DNA splice switching oligonucleotides. | ||
Hum Mol Genet Serum proteomic profiling reveals fragments of MYOM3 as potential biomarkers for monitoring the outcome of therapeutic interventions in muscular dystrophies. | ||
Int J Mol Sci Co-Administration of Simvastatin Does Not Potentiate the Benefit of Gene Therapy in the mdx Mouse Model for Duchenne Muscular Dystrophy. | ||
Stem Cell Reports A Human Stem Cell Model of Fabry Disease Implicates LIMP-2 Accumulation in Cardiomyocyte Pathology. |
