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验证数据展示

  • WB analysis of mouse heart using 22084-1-AP

    WB analysis of mouse heart using 22084-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 22084-1-AP (MYOM1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 22084-1-AP

    IHC staining of mouse heart using 22084-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22084-1-AP (MYOM1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 22084-1-AP

    IHC staining of mouse heart using 22084-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22084-1-AP (MYOM1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MYOM1 Polyclonal antibody

MYOM1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22084-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

MYOM1, Myomesin 1, myomesin 1, 185kDa, Myomesin family member 1, SKELEMIN

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse heart tissue, rat heart tissue
Positive IHC detected inmouse heart tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22084-1-AP targets MYOM1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag17263

Product name: Recombinant human MYOM1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1230-1589 aa of BC116183

Sequence: TNHSTVVLVGDVFKKLQKEAEFQRQEWIRKQGPHFVEYLSWEVTGECNVLLKCKVANIKKETHIVWYKDEREISVDEKHDFKDGICTLLITEFSKKDAGIYEVILKDDRGKDKSRLKLVDEAFKELMMEVCKKIALSATDLKIQSTAEGIQLYSFVTYYVEDLKVNWSHNGSAIRYSDRVKTGVTGEQIWLQINEPTPNDKGKYVMELFDGKTGHQKTVDLSGQAYDEAYAEFQRLKQAAIAEKNRARVLGGLPDVVTIQEGKALNLTCNVWGDPPPEVSWLKNEKALASDDHCNLKFEAGRTAYFTINGVSTADSGKYGLVVKNKYGSETSDFTVSVFIPEEEARMAALESLKGGKKAK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myomesin 1, 185kDa
别名 MYOM1, Myomesin 1, myomesin 1, 185kDa, Myomesin family member 1, SKELEMIN
计算分子量 1685 aa, 188 kDa
观测分子量 170-180 kDa
GenBank蛋白编号BC116183
基因名称 MYOM1
Gene ID (NCBI) 8736
RRIDAB_2878989
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP52179
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYOM1 is a major component of the vertebrate myofibrillar M band. MYOM1 binds myosin, titin, and light meromyosin. This binding is dose-dependent.

实验方案

Product Specific Protocols
WB protocol for MYOM1 antibody 22084-1-APDownload protocol
IHC protocol for MYOM1 antibody 22084-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols