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验证数据展示

  • WB analysis of mouse heart using 22084-1-AP

    WB analysis of mouse heart using 22084-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 22084-1-AP (MYOM1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 22084-1-AP

    IHC staining of mouse heart using 22084-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22084-1-AP (MYOM1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 22084-1-AP

    IHC staining of mouse heart using 22084-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22084-1-AP (MYOM1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MYOM1 Polyclonal antibody

MYOM1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22084-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

MYOM1, Myomesin 1, myomesin 1, 185kDa, Myomesin family member 1, SKELEMIN

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse heart tissue, rat heart tissue
Positive IHC detected inmouse heart tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22084-1-AP targets MYOM1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MYOM1 fusion protein Ag17263 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myomesin 1, 185kDa
别名 MYOM1, Myomesin 1, myomesin 1, 185kDa, Myomesin family member 1, SKELEMIN
计算分子量 1685 aa, 188 kDa
观测分子量 170-180 kDa
GenBank蛋白编号BC116183
基因名称 MYOM1
Gene ID (NCBI) 8736
RRIDAB_2878989
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP52179
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYOM1 is a major component of the vertebrate myofibrillar M band. MYOM1 binds myosin, titin, and light meromyosin. This binding is dose-dependent.

实验方案

Product Specific Protocols
WB protocol for MYOM1 antibody 22084-1-APDownload protocol
IHC protocol for MYOM1 antibody 22084-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols