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Myoferlin Polyclonal antibody

Myoferlin Polyclonal Antibody for FC, IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 19548-1-AP

Print datasheet

Synonyms

Fer 1 like protein 3, FER1L3, KIAA1207, MYOF, Myoferlin

验证数据展示

  • WB analysis using 19548-1-AP

    WB analysis using 19548-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19548-1-AP (Myoferlin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 19548-1-AP

    IHC staining of human placenta using 19548-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19548-1-AP (Myoferlin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 19548-1-AP

    IHC staining of human placenta using 19548-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19548-1-AP (Myoferlin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HeLa using 19548-1-AP

    FC experiment of HeLa using 19548-1-AP

    1X10^6 HeLa cells were stained with 0.2ug Myoferlin antibody (19548-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

  • IF Staining of HeLa using 19548-1-AP

    IF Staining of HeLa using 19548-1-AP

    Immunofluorescent analysis of Hela cells, using MYOF antibody 19548-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inA549 cells, human placenta tissue, HeLa cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells
Positive FC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

19548-1-AP targets Myoferlin in WB, IF, FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications FC, IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myoferlin
Synonyms Fer 1 like protein 3, FER1L3, KIAA1207, MYOF, Myoferlin
Calculated Molecular Weight 235 kDa
Observed Molecular Weight 235-250 kDa
GenBank Accession NumberNM_133337
Gene Symbol MYOF
Gene ID (NCBI) 26509
RRIDAB_10642558
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NZM1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYOF, also named as FER1L3 and KIAA1207, belongs to the ferlin family. MYOF is a calcium/phospholipid-binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. It is involved in endocytic recycling. MYOF is implicated in VEGF signal transduction by regulating the levels of the receptor KDR. The antibody can recognize all the 8 isoforms of MYOF.

实验方案

Product Specific Protocols
WB protocol for Myoferlin antibody 19548-1-APDownload protocol
IHC protocol for Myoferlin antibody 19548-1-APDownload protocol
IF protocol for Myoferlin antibody 19548-1-APDownload protocol
FC protocol for Myoferlin antibody 19548-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Proteomics

Quantitative proteomics identifies myoferlin as a novel regulator of A Disintegrin and Metalloproteinase 12 in HeLa cells.

Authors - Yanqing Zhou
View Article
humanWB

Eur J Med Chem

Discovery of potent thiazolidin-4-one sulfone derivatives for inhibition of proliferation of osteosarcoma in vitro and in vivo

Authors - Xuwen Chen
View Article