MYOD1 Polyclonal antibody

MYOD1 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

FC, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 18943-1-AP

Print datasheet

Synonyms

bHLHc1, Myf 3, MYF3, MYOD, MYOD1, myogenic differentiation 1, Myogenic factor 3, PUM



经过测试的应用

Positive WB detected inmouse heart tissue, human heart tissue, mouse skeletal muscle tissue, rat heart tissue
Positive IHC detected inmouse embryo tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inC2C12 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

产品信息

18943-1-AP targets MYOD1 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IHC, WB, ELISA
Cited Applications IF, IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, goat, pig
Immunogen MYOD1 fusion protein Ag13512 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myogenic differentiation 1
Synonyms bHLHc1, Myf 3, MYF3, MYOD, MYOD1, myogenic differentiation 1, Myogenic factor 3, PUM
Calculated molecular weight 320 aa, 35 kDa
Observed molecular weight 35-45 kDa
GenBank accession numberBC064493
Gene symbol MYOD1
UniprotP15172
Gene ID (NCBI) 4654
RRIDAB_10603467
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYOD1, also named as BHLHC1 or MYF3, is a 320 amino acid protein, which promotes the transcriptional activity of MYOD1 through its CDK9-mediated phosphorylation. This phosphorylation promotes its function in muscle differentiation. MYOD1 acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. MYOD1 together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. he calcualted molecular weight of MYOD1 is 34 kDa, but modified MYOD1 is about 45 kDa. (PMID: 12037670)

实验方案

Product Specific Protocols
WB protocol for MYOD1 antibody 18943-1-APDownload protocol
IHC protocol for MYOD1 antibody 18943-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nature

A plant-derived natural photosynthetic system for improving cell anabolism

Authors - Pengfei Chen
mouseIHC

Nature

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Authors - Joshua E Burda
WB

Nature

SAR1B senses leucine levels to regulate mTORC1 signalling.

Authors - Jie Chen
mouseWB

Nat Commun

The transcription factor NF-Y participates to stem cell fate decision and regeneration in adult skeletal muscle.

Authors - Giovanna Rigillo
mouseWB

J Cachexia Sarcopenia Muscle

circRNAome profiling reveals circFgfr2 regulates myogenesis and muscle regeneration via a feedback loop.

Authors - Junyu Yan
humanWB,IHC

Cancer Res

YAP1-mediated suppression of USP31 enhances NF-κB activity to promote sarcomagenesis.

Authors - Shuai Ye