|Positive WB detected in||mouse heart tissue, human heart tissue, mouse skeletal muscle tissue, rat heart tissue|
|Positive IHC detected in||mouse embryo tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||C2C12 cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
18943-1-AP targets MYOD1 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Applications||FC, IHC, WB, ELISA|
|Cited Applications||IF, IHC, WB|
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, chicken, goat, pig|
|Immunogen||MYOD1 fusion protein Ag13512|
|Host / Isotype||Rabbit / IgG|
|Full Name||myogenic differentiation 1|
|Synonyms||bHLHc1, Myf 3, MYF3, MYOD, MYOD1, myogenic differentiation 1, Myogenic factor 3, PUM|
|Calculated molecular weight||320 aa, 35 kDa|
|Observed molecular weight||35-45 kDa|
|GenBank accession number||BC064493|
|Gene ID (NCBI)||4654|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
MYOD1, also named as BHLHC1 or MYF3, is a 320 amino acid protein, which promotes the transcriptional activity of MYOD1 through its CDK9-mediated phosphorylation. This phosphorylation promotes its function in muscle differentiation. MYOD1 acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. MYOD1 together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. he calcualted molecular weight of MYOD1 is 34 kDa, but modified MYOD1 is about 45 kDa. (PMID: 12037670)
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