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MYO9B Polyclonal antibody

MYO9B Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 12432-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA and More (1)

Unconventional myosin-IXb, Unconventional myosin-9b, MYR5, myosin IXB, MYO9B variant protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse thymus tissue, HL-60 cells, Jurkat cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MYO9B Ready-To-Use IHC Kit. MYO9B primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12432-1-AP targets MYO9B in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 MYO9B fusion protein Ag3108 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myosin IXB
别名 Unconventional myosin-IXb, Unconventional myosin-9b, MYR5, myosin IXB, MYO9B variant protein
计算分子量 2023 aa, 223 kDa
观测分子量 230-250 kDa
GenBank蛋白编号BC018108
基因名称 MYO9B
Gene ID (NCBI) 4650
RRIDAB_2148635
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13459
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MYO9B antibody 12432-1-APDownload protocol
IHC protocol for MYO9B antibody 12432-1-APDownload protocol
IP protocol for MYO9B antibody 12432-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Commun

Myo9b mutations are associated with altered dendritic cell functions and increased susceptibility to autoimmune diabetes onset

Authors - Jing Zhang
human,mouseWB,IF

Nat Commun

An allosteric inhibitor of RhoGAP class-IX myosins suppresses the metastatic features of cancer cells

Authors - Despoina Kyriazi
  • KD Validated
humanWB

J Thromb Haemost

The RhoA regulators Myo9b and GEF-H1 are targets of cyclic nucleotide-dependent kinases in platelets.

Authors - Shane Comer
humanWB,IF

Cell Rep

Specific Myosins Control Actin Organization, Cell Morphology, and Migration in Prostate Cancer Cells.

Authors - Katarzyna A Makowska
  • KD Validated
mouseIF

J Bone Miner Res

The RhoGAP Myo9b Promotes Bone Growth by Mediating Osteoblastic Responsiveness to IGF-1.

Authors - Brooke K McMichael
  • KO Validated
mouseWB

J Immunol

TSAd Plays a Major Role in Myo9b-Mediated Suppression of Malignant Pleural Effusion by Regulating TH1/TH17 Cell Response.

Authors - Feng-Shuang Yi
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