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验证数据展示

  • IHC staining of mouse eye using 28562-1-AP

    IHC staining of mouse eye using 28562-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 28562-1-AP (MYO3A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYO3A antibody included
  • IHC staining of mouse eye using 28562-1-AP

    IHC staining of mouse eye using 28562-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 28562-1-AP (MYO3A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYO3A antibody included
  • IF Staining of mouse eye using 28562-1-AP

    IF Staining of mouse eye using 28562-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using MYO3A antibody (28562-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse eye using 28562-1-AP

    IF Staining of mouse eye using 28562-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using MYO3A antibody (28562-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

MYO3A Polyclonal antibody

MYO3A Polyclonal Antibody for IHC, IF-P, ELISA
Cat No. 28562-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

IHC, IF-P, ELISA

别名

DFNB30, MYO3A, myosin IIIA

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse eye tissue
Planning an IHC experiment? We recommend our IHCeasy MYO3A Ready-To-Use IHC Kit. MYO3A primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28562-1-AP targets MYO3A in IHC, IF-P, ELISA applications and shows reactivity with mouse samples.

经测试应用 IHC, IF-P, ELISA Application Description
经测试反应性 mouse
免疫原

CatNo: Ag29335

Product name: Recombinant human MYO3A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1090-1308 aa of NM_017433

Sequence: QSAARGHLVRKQRKEIVDMKNTAVTTIQTSDQEFDYKKNFENTRESFVKKQAENAISANERFISAPNNKGSVSVVKTSTFKPEEETTNAVESNNRVYQTPKKMNNVYEEEVKQEFYLVGPEVSPKQKSVKDLEENSNLRKVEKEEAMIQSYYQRYTEERNCEESKAAYLERKAISERPSYPVPWLAENETSFKKTLEPTLSQRSIYQNANSMEKEKKTS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myosin IIIA
别名 DFNB30, MYO3A, myosin IIIA
计算分子量 186 kDa
观测分子量190 kda
GenBank蛋白编号NM_017433
基因名称 MYO3A
Gene ID (NCBI) 53904
RRIDAB_3086065
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NEV4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for MYO3A antibody 28562-1-APDownload protocol
IHC protocol for MYO3A antibody 28562-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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