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验证数据展示

  • WB analysis using 28841-1-AP

    WB analysis using 28841-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28841-1-AP (MYO1F antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of THP-1 using 28841-1-AP

    IF Staining of THP-1 using 28841-1-AP

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using MYO1F antibody (28841-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

MYO1F Polyclonal antibody

MYO1F Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 28841-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

Unconventional myosin-If, Myosin-Ie, myosin IF, Myosin Ie

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, rat spleen tissue
Positive IF/ICC detected inTHP-1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28841-1-AP targets MYO1F in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MYO1F fusion protein Ag29899 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myosin IF
别名 Unconventional myosin-If, Myosin-Ie, myosin IF, Myosin Ie
计算分子量 1098 aa, 125 kDa
观测分子量 115-125 kDa
GenBank蛋白编号BC028071
基因名称 MYO1F
Gene ID (NCBI) 4542
RRIDAB_3669671
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00160
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Myosin family members are proteins that hydrolyze ATP to generate force or directional movement along actin filaments and are implicated in the rearrangement of the cytoskeleton in several cell types. Myosin 1F (MYO1F), a long tailed class I myosin, is highly expressed in natural killer (NK) cells, macrophages, dendritic cells, and neutrophils. MYO1F plays a critical role in the mediating of signaling molecules "trafficking from membrane to cytoplasm," and this process is essential for the antifungal signaling pathway activation.

实验方案

Product Specific Protocols
WB protocol for MYO1F antibody 28841-1-APDownload protocol
IF protocol for MYO1F antibody 28841-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols