验证数据展示
经过测试的应用
| Positive WB detected in | mouse lung tissue | 
| Positive IP detected in | mouse lung tissue | 
推荐稀释比
| 应用 | 推荐稀释比 | 
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 | 
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below | 
| IHC | See 2 publications below | 
产品信息
14028-1-AP targets MYO1D in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, ELISA Application Description | 
| 文献引用应用 | WB, IHC | 
| 经测试反应性 | human, mouse | 
| 文献引用反应性 | human | 
| 免疫原 | CatNo: Ag5176 Product name: Recombinant human MYO1D protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 1-350 aa of BC030602 Sequence: MAEQESLEFGKADFVLMDTVSMPEFMANLRLRFEKGRIYTFIGEVVVSVNPYKLLNIYGRDTIEQYKGRELYERPPHLFAIADAAYKAMKRRSKDTCIVISGESGAGKTEASKYIMQYIAAITNPSQRAEVERVKNMLLKSNCVLEAFGNAKTNRNDNSSRFGKYMDINFDFKGDPIGGHINNYLLEKSRVIVQQPGERSFHSFYQLLQGGSEQMLRSLHLQKSLSSYNYIHVGAQLKSSINDAAEFRVVADAMKVIGFKPEEIQTVYKILAAILHLGNLKFVVDGDTPLIENGKVVSIIAELLSTKTDMVEKALLYRTVATGRDIIDKQHTEQEASYGRDAFAKAIYER种属同源性预测 | 
| 宿主/亚型 | Rabbit / IgG | 
| 抗体类别 | Polyclonal | 
| 产品类型 | Antibody | 
| 全称 | myosin ID | 
| 别名 | KIAA0727, MYO1D, myosin ID, myr4 | 
| 计算分子量 | 116 kDa | 
| 观测分子量 | 100 kDa | 
| GenBank蛋白编号 | BC030602 | 
| 基因名称 | MYO1D | 
| Gene ID (NCBI) | 4642 | 
| RRID | AB_2266818 | 
| 偶联类型 | Unconjugated | 
| 形式 | Liquid | 
| 纯化方式 | Antigen affinity purification | 
| UNIPROT ID | O94832 | 
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. | 
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. | 
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for MYO1D antibody 14028-1-AP | Download protocol | 
| WB protocol for MYO1D antibody 14028-1-AP | Download protocol | 
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols | 
发表文章
| Species | Application | Title | 
|---|---|---|
| Transl Oncol Identification of metastasis-associated exoDEPs in colorectal cancer using label-free proteomics. | ||
| Cytoskeleton (Hoboken) Myosin VI is required for maintenance of brush border structure, composition, and membrane trafficking functions in the intestinal epithelial cell. | 



