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Host
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Species Reactivity
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Conjugate
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验证数据展示

  • WB analysis of mouse lung using 14028-1-AP

    WB analysis of mouse lung using 14028-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 14028-1-AP (MYO1D antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse lung using 14028-1-AP

    IP experiment of mouse lung using 14028-1-AP

    IP result of anti-MYO1D (IP:14028-1-AP, 4ug; Detection:14028-1-AP 1:300) with mouse lung tissue lysate 4000ug.

MYO1D Polyclonal antibody

MYO1D Polyclonal Antibody for WB, IP, ELISA
Cat No. 14028-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, ELISA and More (1)

别名

KIAA0727, MYO1D, myosin ID, myr4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse lung tissue
Positive IP detected inmouse lung tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14028-1-AP targets MYO1D in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse
文献引用反应性human
免疫原

CatNo: Ag5176

Product name: Recombinant human MYO1D protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-350 aa of BC030602

Sequence: MAEQESLEFGKADFVLMDTVSMPEFMANLRLRFEKGRIYTFIGEVVVSVNPYKLLNIYGRDTIEQYKGRELYERPPHLFAIADAAYKAMKRRSKDTCIVISGESGAGKTEASKYIMQYIAAITNPSQRAEVERVKNMLLKSNCVLEAFGNAKTNRNDNSSRFGKYMDINFDFKGDPIGGHINNYLLEKSRVIVQQPGERSFHSFYQLLQGGSEQMLRSLHLQKSLSSYNYIHVGAQLKSSINDAAEFRVVADAMKVIGFKPEEIQTVYKILAAILHLGNLKFVVDGDTPLIENGKVVSIIAELLSTKTDMVEKALLYRTVATGRDIIDKQHTEQEASYGRDAFAKAIYER

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myosin ID
别名 KIAA0727, MYO1D, myosin ID, myr4
计算分子量 116 kDa
观测分子量 100 kDa
GenBank蛋白编号BC030602
基因名称 MYO1D
Gene ID (NCBI) 4642
RRIDAB_2266818
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO94832
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MYO1D antibody 14028-1-APDownload protocol
IP protocol for MYO1D antibody 14028-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Transl Oncol

Identification of metastasis-associated exoDEPs in colorectal cancer using label-free proteomics.

Authors - Xinlu Liu
View Article
humanIHC

Cytoskeleton (Hoboken)

Myosin VI is required for maintenance of brush border structure, composition, and membrane trafficking functions in the intestinal epithelial cell.

Authors - Hegan Peter S PS
View Article