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验证数据展示

  • WB analysis of HepG2 using 17499-1-AP

    WB analysis of HepG2 using 17499-1-AP

    WB result of MYO1A antibody (17499-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYO1A transfected HepG2 cells.

  • WB analysis of mouse brain using 17499-1-AP

    WB analysis of mouse brain using 17499-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17499-1-AP (MYO1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 17499-1-AP

    IP experiment of HeLa using 17499-1-AP

    IP result of anti-MYO1A (IP:17499-1-AP, 4ug; Detection:17499-1-AP 1:500) with HeLa cells lysate 3200ug.

  • IHC staining of human colon cancer using 17499-1-AP

    IHC staining of human colon cancer using 17499-1-AP

    Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 17499-1-AP (MYO1A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYO1A antibody included
  • IF Staining of HepG2 using 17499-1-AP

    IF Staining of HepG2 using 17499-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MYO1A antibody (17499-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Featured Product
  • KD/KO验证

MYO1A Polyclonal antibody

MYO1A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 17499-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

MIHC, DFNA48, Brush border myosin I, BBM-I, BBMI

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, HepG2 cells, HeLa cells, A431 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy MYO1A Ready-To-Use IHC Kit. MYO1A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

17499-1-AP targets MYO1A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
免疫原 MYO1A fusion protein Ag11682 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myosin IA
别名 MIHC, DFNA48, Brush border myosin I, BBM-I, BBMI
计算分子量 1043 aa, 118 kDa
观测分子量 130 kDa
GenBank蛋白编号BC059387
基因名称 MYO1A
Gene ID (NCBI) 4640
RRIDAB_2148172
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UBC5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MYO1A antibody 17499-1-APDownload protocol
IHC protocol for MYO1A antibody 17499-1-APDownload protocol
IF protocol for MYO1A antibody 17499-1-APDownload protocol
IP protocol for MYO1A antibody 17499-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Cell Rep Med

Personalized drug screening using patient-derived organoid and its clinical relevance in gastric cancer

Authors - Yi Zhao
View Article