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  • Featured Product
  • KD/KO Validated

MYO19 Polyclonal antibody

MYO19 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, monkey, rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 23906-1-AP

Print datasheet

Synonyms

MYO19, MYOHD1, myosin XIX

验证数据展示

  • WB analysis of HeLa using 23906-1-AP

    WB analysis of HeLa using 23906-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 23906-1-AP (MYO19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 23906-1-AP

    WB analysis of A549 using 23906-1-AP

    WB result of MYO19 antibody (23906-1-AP, 1:300) with si-Control and si-MYO19 transfected A549 cells.

  • IF Staining of A549 using 23906-1-AP

    IF Staining of A549 using 23906-1-AP

    Immunofluorescent analysis of A549 cells using 23906-1-AP (MYO19 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human kidney using 23906-1-AP

    IHC staining of human kidney using 23906-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23906-1-AP (MYO19 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYO19 antibody included
  • IHC staining of rat kidney using 23906-1-AP

    IHC staining of rat kidney using 23906-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 23906-1-AP (MYO19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYO19 antibody included
  • IHC staining of rat testis using 23906-1-AP

    IHC staining of rat testis using 23906-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 23906-1-AP (MYO19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYO19 antibody included

经过测试的应用

Positive WB detected inHeLa cells, A549 cells, COS-7 cells
Positive IHC detected inhuman kidney tissue, rat kidney tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy MYO19 Ready-To-Use IHC Kit. MYO19 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23906-1-AP targets MYO19 in WB, IF, IHC, ELISA applications and shows reactivity with human, monkey, rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity human, monkey, rat
Immunogen MYO19 fusion protein Ag20995 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myosin XIX
Synonyms MYO19, MYOHD1, myosin XIX
Calculated Molecular Weight 970 aa, 109 kDa
Observed Molecular Weight 109 kDa
GenBank Accession NumberBC008900
Gene Symbol MYO19
Gene ID (NCBI) 80179
RRIDAB_2879357
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96H55
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYO19 is also named as MYOHD1. It is Actin-based motor molecule with ATPase activity that localizes to the mitochondrion outer membrane (PubMed:19932026, PubMed:23568824, PubMed:25447992) and required for mitochondrial inheritance during mitosis (PubMed:25447992). MYO19 can enable actin binding activity and be involved in regulation of cytokinesis and regulation of mitochondrial fission. MYO19 can be detected as about 109 kDa.

实验方案

Product Specific Protocols
WB protocol for MYO19 antibody 23906-1-APDownload protocol
IHC protocol for MYO19 antibody 23906-1-APDownload protocol
IF protocol for MYO19 antibody 23906-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols