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验证数据展示

  • WB analysis of HeLa using 23906-1-AP

    WB analysis of HeLa using 23906-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 23906-1-AP (MYO19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 23906-1-AP

    WB analysis of A549 using 23906-1-AP

    WB result of MYO19 antibody (23906-1-AP, 1:300) with si-Control and si-MYO19 transfected A549 cells.

  • IF Staining of A549 using 23906-1-AP

    IF Staining of A549 using 23906-1-AP

    Immunofluorescent analysis of A549 cells using 23906-1-AP (MYO19 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human kidney using 23906-1-AP

    IHC staining of human kidney using 23906-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23906-1-AP (MYO19 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYO19 antibody included
  • IHC staining of rat kidney using 23906-1-AP

    IHC staining of rat kidney using 23906-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 23906-1-AP (MYO19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYO19 antibody included
  • IHC staining of rat testis using 23906-1-AP

    IHC staining of rat testis using 23906-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 23906-1-AP (MYO19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYO19 antibody included
  • Featured Product
  • KD/KO验证

MYO19 Polyclonal antibody

MYO19 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 23906-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, monkey, rat and More (1)

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

MYO19, MYOHD1, myosin XIX

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, A549 cells, COS-7 cells
Positive IHC detected inhuman kidney tissue, rat kidney tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy MYO19 Ready-To-Use IHC Kit. MYO19 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23906-1-AP targets MYO19 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, monkey, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, monkey, rat
文献引用反应性human, mouse
免疫原

CatNo: Ag20995

Product name: Recombinant human MYO19 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 441-770 aa of BC008900

Sequence: EACGLVETIHISAAGFPIRVSHRNFVERYKLLRRLHPCTSSGPDSPYPAKGLPEWCPHSEEATLEPLIQDILHTLPVLTQAAAITGDSAEAMPAPMHCGRTKVFMTDSMLELLECGRARVLEQCARCIQGGWRRHRHREQERQWRAVMLIQAAIRSWLTRKHIQRLHAAATVIKRAWQKWRIRMACLAAKELDGVEEKHFSQAPCSLSTSPLQTRLLEAIIRLWPLGLVLANTAMGVGSFQRKLVVWACLQLPRGSPSSYTVQTAQDQAGVTSIRALPQGSIKFHCRKSPLRYADICPEPSPYSITGFNQILLERHRLIHVTSSAFTGLG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myosin XIX
别名 MYO19, MYOHD1, myosin XIX
计算分子量 970 aa, 109 kDa
观测分子量 109 kDa
GenBank蛋白编号BC008900
基因名称 MYO19
Gene ID (NCBI) 80179
RRIDAB_2879357
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96H55
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYO19 is also named as MYOHD1. It is Actin-based motor molecule with ATPase activity that localizes to the mitochondrion outer membrane (PubMed:19932026, PubMed:23568824, PubMed:25447992) and required for mitochondrial inheritance during mitosis (PubMed:25447992). MYO19 can enable actin binding activity and be involved in regulation of cytokinesis and regulation of mitochondrial fission. MYO19 can be detected as about 109 kDa.

实验方案

Product Specific Protocols
IF protocol for MYO19 antibody 23906-1-APDownload protocol
IHC protocol for MYO19 antibody 23906-1-APDownload protocol
WB protocol for MYO19 antibody 23906-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Sci Adv

ARMC1 partitions between distinct complexes and assembles MIRO with MTFR to control mitochondrial distribution

Authors - Michael J McKenna
View Article
human,mouseWB,IHC,IF

J Pharm Anal

Coral calcium hydride promotes peripheral mitochondrial division and reduces AT-II cells damage in ARDS via activation of the Trx2/Myo19/Drp1 pathway

Authors - Qian Li
View Article
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