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验证数据展示

  • WB analysis of Jurkat using 14611-1-AP (same clone as 14611-1-PBS)

    WB analysis of Jurkat using 14611-1-AP (same clone as 14611-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14611-1-AP (MYO18A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14611-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 14611-1-AP (same clone as 14611-1-PBS)

    IP experiment of HeLa using 14611-1-AP (same clone as 14611-1-PBS)

    IP result of anti-MYO18A (IP:14611-1-AP, 3ug; Detection:14611-1-AP 1:300) with HeLa cells lysate 3000ug. This data was developed using the same antibody clone with 14611-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 14611-1-AP (same clone as 14611-1-PBS)

    IHC staining of mouse skeletal muscle using 14611-1-AP (same clone as 14611-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14611-1-AP (MYO18A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14611-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYO18A antibody included
  • IHC staining of mouse heart using 14611-1-AP (same clone as 14611-1-PBS)

    IHC staining of mouse heart using 14611-1-AP (same clone as 14611-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14611-1-AP (MYO18A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14611-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYO18A antibody included
  • IHC staining of mouse heart using 14611-1-AP (same clone as 14611-1-PBS)

    IHC staining of mouse heart using 14611-1-AP (same clone as 14611-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14611-1-AP (MYO18A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14611-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYO18A antibody included
  • Featured Product
  • KD/KO Validated

MYO18A Polyclonal antibody, PBS Only

MYO18A Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 14611-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

CD245, KIAA0216, Molecule associated with JAK3 N-terminus, myosin XVIIIA, myosinXVIIIA

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy MYO18A Ready-To-Use IHC Kit. MYO18A primary antibody included.

产品信息

14611-1-PBS targets MYO18A in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6185

Product name: Recombinant human MYO18A protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1601-2002 aa of BC039612

Sequence: TLSDQVNRRDFESEKRLRKDLKRTKALLADAQLMLDHLKNSAPSKREIAQLKNQLEESEFTCAAAVKARKAMEVEIEDLHLQIDDIAKAKTALEEQLSRLQREKNEIQNRLEEDQEDMNELMKKHKAAVAQASRDLAQINDLQAQLEEANKEKQELQEKLQALQSQVEFLEQSMVDKSLVSRQEAKIRELETRLEFERTQVKRLESLASRLKENMEKLTEERDQRIAAENREKEQNKRLQRQLRDTKEEMGELARKEAEASRKKHELEMDLESLEAANQSLQADLKLAFKRIGDLQAAIEDEMESDENEDLINSEGDSDVDSELEDRVDGVKSWLSKNKGPSKAASDDGSLKSSSPTSYWKSLAPDRSDDEHDPLDNTSRPRYSHSYLSDSDTEAKLTETNA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myosin XVIIIA
别名 CD245, KIAA0216, Molecule associated with JAK3 N-terminus, myosin XVIIIA, myosinXVIIIA
计算分子量 233 kDa
观测分子量 230 kDa, 190 kDa
GenBank蛋白编号BC039612
基因名称 MYO18A
Gene ID (NCBI) 399687
RRIDAB_2201447
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92614
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MYO18A is an unconventional myosin that may play a role in the stabilization and organization of the actin cytoskeleton. Recently MYO18A has been identified as the receptor of SP and is responsible for clearance of SP-A-opsonized bacteria in in vivo infection. Two different isoforms of mouse Myo18A have been identified: Myo18A-alpha and Myo18A-beta as 230- and 190-kDa proteins, respectively. Myo18a-alpha colocalizes with the ER-Golgi complex and with membrane ruffles and filopodia, while Myo18a-beta localizes diffusely to the cytoplasm.

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