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MYO16 Polyclonal antibody

MYO16 Polyclonal Antibody for IF/ICC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, ELISA

Conjugate

Unconjugated

Cat No : 18828-1-AP

Print datasheet

Synonyms

KIAA0865, MYO16, Myo16b, myosin XVI, MYR8, Unconventional myosin 16

验证数据展示

  • IHC staining of human bladder using 18828-1-AP

    IHC staining of human bladder using 18828-1-AP

    Immunohistochemical analysis of paraffin-embedded human bladder using 18828-1-AP (MYO16 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with MYO16 antibody included
  • IF Staining of A431 using 18828-1-AP

    IF Staining of A431 using 18828-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed A431 cells using 18828-1-AP (MYO16 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IHC detected inhuman bladder tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy MYO16 Ready-To-Use IHC Kit. MYO16 primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18828-1-AP targets MYO16 in IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IHC, ELISA
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myosin XVI
Synonyms KIAA0865, MYO16, Myo16b, myosin XVI, MYR8, Unconventional myosin 16
Calculated Molecular Weight 1858 aa, 206 kDa
Observed Molecular Weight 123 kDa, 210 kDa, 106 kDa
GenBank Accession NumberBC146791
Gene Symbol MYO16
Gene ID (NCBI) 23026
RRIDAB_10858634
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6X6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYO16, also named as KIAA0865, MYO16B, is actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. MYO16 may be involved in targeting of the catalytic subunit of protein phosphatase 1 during brain development. The antibody can recognize isoform1, isoform2 and isoform3 of MYO16.

实验方案

Product Specific Protocols
IHC protocol for MYO16 antibody 18828-1-APDownload protocol
IF protocol for MYO16 antibody 18828-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols