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验证数据展示

  • WB analysis using 13369-1-AP

    WB analysis using 13369-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13369-1-AP (MYNN antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 13369-1-AP

    IHC staining of human placenta using 13369-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13369-1-AP (MYNN antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MYNN Polyclonal antibody

MYNN Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13369-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

MYNN, myoneurin, OSZF, SBBIZ1, ZBTB31

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inCOLO 320 cells, Caco-2 cells, mouse brain tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13369-1-AP targets MYNN in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag4188

Product name: Recombinant human MYNN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 303-610 aa of BC033620

Sequence: MCNTCGKVFSEASSLRRHMRIHKGVKPYVCHLCGKAFTQCNQLKTHVRTHTGEKPYKCELCDKGFAQKCQLVFHSRMHHGEEKPYKCDVCNLQFATSSNLKIHARKHSGEKPYVCDRCGQRFAQASTLTYHVRRHTGEKPYVCDTCGKAFAVSSSLITHSRKHTGEKPYICGICGKSFISSGELNKHFRSHTGERPFICELCGNSYTDIKNLKKHKTKVHSGADKTLDSSAEDHTLSEQDSIQKSPLSETMDVKPSDMTLPLALPLGTEDHHMLLPVTDTQSPTSDTLLRSTVNGYSEPQLIFLQQLY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myoneurin
别名 MYNN, myoneurin, OSZF, SBBIZ1, ZBTB31
计算分子量 610 aa, 69 kDa
观测分子量 69 kDa
GenBank蛋白编号BC033620
基因名称 MYNN
Gene ID (NCBI) 55892
RRIDAB_2923559
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NPC7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Myoneurin (MYNN) gene locates on 3q26.2 and encodes a member of the BTB/POZ and zinc finger (ZF) domain-containing protein family that is involved in the control of gene expression. MYNN is widely expressed in various tissue types in mammals. MYNN has 4 isoforms with the molecular mass of 10, 57, 65-69 kDa. (PMID: 30120764)

实验方案

Product Specific Protocols
IHC protocol for MYNN antibody 13369-1-APDownload protocol
WB protocol for MYNN antibody 13369-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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