验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, L02 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human heart tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24309-1-AP targets MYLK4 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag19314 Product name: Recombinant human MYLK4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 4-110 aa of BC132831 Sequence: VKRLEEFNTCYNSNQLEKMAFFQCREEVEKVKCFLEKNSGDQDSRSRHNEAKEVWSNADLTERMPVKSKRTSALAVDIPAPPAPFDHRIVTAKQGAVNSFYTVSKTE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | myosin light chain kinase family, member 4 |
| 别名 | MLCK4, MYLK4, SgK085, Sugen kinase 85 |
| 计算分子量 | 388 aa, 45 kDa |
| 观测分子量 | 45 kDa |
| GenBank蛋白编号 | BC132831 |
| 基因名称 | MYLK4 |
| Gene ID (NCBI) | 340156 |
| RRID | AB_2877632 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q86YV6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MYLK4(Myosin light chain kinase family member 4) is also named as SGK085 and belongs to the CAMK Ser/Thr protein kinase family. MLCKs are activated by Ca2+-calmodulin complexes and require Mg2+ or Ca2+ as a co-factor. It has 2 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MYLK4 antibody 24309-1-AP | Download protocol |
| IHC protocol for MYLK4 antibody 24309-1-AP | Download protocol |
| IP protocol for MYLK4 antibody 24309-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A Cardiac myosin light chain is phosphorylated by Ca2+/calmodulin-dependent and -independent kinase activities. | ||
J Exp Clin Cancer Res MYLK4 promotes tumor progression through the activation of epidermal growth factor receptor signaling in osteosarcoma.
| ||
bioRxiv GATA1-defective immune-megakaryocytes as possible drivers of idiopathic pulmonary fibrosis |
