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- KD/KO Validated
MYLK4 Polyclonal antibody
MYLK4 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, L02 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human heart tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
The immunogen of 24309-1-AP is MYLK4 Fusion Protein expressed in E. coli.
| Tested Applications | IHC, IP, WB, ELISA |
| Cited Applications | WB, IF |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | MYLK4 fusion protein Ag19314 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | myosin light chain kinase family, member 4 |
| Synonyms | MLCK4, MYLK4, SgK085, Sugen kinase 85 |
| Calculated Molecular Weight | 388 aa, 45 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC132831 |
| Gene Symbol | MYLK4 |
| Gene ID (NCBI) | 340156 |
| RRID | AB_2877632 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86YV6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MYLK4(Myosin light chain kinase family member 4) is also named as SGK085 and belongs to the CAMK Ser/Thr protein kinase family. MLCKs are activated by Ca2+-calmodulin complexes and require Mg2+ or Ca2+ as a co-factor. It has 2 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MYLK4 antibody 24309-1-AP | Download protocol |
| IHC protocol for MYLK4 antibody 24309-1-AP | Download protocol |
| IP protocol for MYLK4 antibody 24309-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Exp Clin Cancer Res MYLK4 promotes tumor progression through the activation of epidermal growth factor receptor signaling in osteosarcoma.
| ||
bioRxiv GATA1-defective immune-megakaryocytes as possible drivers of idiopathic pulmonary fibrosis |
