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验证数据展示

  • WB analysis using 21173-1-AP (same clone as 21173-1-PBS)

    WB analysis using 21173-1-AP (same clone as 21173-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21173-1-AP (MYLK2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21173-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 21173-1-AP (same clone as 21173-1-PBS)

    IHC staining of mouse skeletal muscle using 21173-1-AP (same clone as 21173-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21173-1-AP (MYLK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21173-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 21173-1-AP (same clone as 21173-1-PBS)

    IHC staining of mouse skeletal muscle using 21173-1-AP (same clone as 21173-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21173-1-AP (MYLK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21173-1-PBS in a different storage buffer formulation.

  • WB analysis using 21173-1-AP (same clone as 21173-1-PBS)

    WB analysis using 21173-1-AP (same clone as 21173-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 21173-1-AP (MYLK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21173-1-PBS in a different storage buffer formulation.

MYLK2 Polyclonal antibody, PBS Only

MYLK2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 21173-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

21173-1-PBS targets MYLK2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag15424

Product name: Recombinant human MYLK2 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-300 aa of BC069627

Sequence: MATENGAVELGIQNPSTDKAPKGPTGERPLAAGKDPGPPDPKKAPDPPTLKKDAKAPASEKGDGTLAQPSTSSQGPKGEGDRGGGPAEGSAGPPAALPQQTATPETSVKKPKAEQGASGSQDPGKPRVGKKAAEGQAAARRGSPAFLHSPSCPAIISSSEKLLAKKPPSEASELTFEGVPMTHSPTDPRPAKAEEGKNILAESQKEVGEKTPGQAGQAKMQGDTSRGIEFQAVPSEKSEVGQALCLTAREEDCFQILDDCPPPPAPFPHRMVELRTGNVSSEFSMNSKEALGGGKFGAVC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myosin light chain kinase 2
别名
计算分子量 596 aa, 65 kDa
观测分子量 65 kDa
GenBank蛋白编号BC069627
基因名称 MYLK2
Gene ID (NCBI) 85366
RRIDAB_10733123
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H1R3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MYLK2, also named as MLCK2, belongs to the protein kinase superfamily and CAMK Ser/Thr protein kinase family. MYLK2 catalyze the reaction: ATP + [myosin light-chain] = ADP + [myosin light-chain] phosphate. MYLK2 is implicated in the level of global muscle contraction and cardiac function. MYLK2 phosphorylates a specific serine in the N-terminus of a myosin light chain. Mutation of MYLK2 will cause the cardiomyopathy familial hypertrophic (CMH).

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