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验证数据展示

  • WB analysis of human cerebellum using 15455-1-AP

    WB analysis of human cerebellum using 15455-1-AP

    human cerebellum tissue were subjected to SDS PAGE followed by western blot with 15455-1-AP (MYLIP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 15455-1-AP

    IHC staining of human testis using 15455-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 15455-1-AP

    IHC staining of human testis using 15455-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 40x lens).

MYLIP Polyclonal antibody

MYLIP Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15455-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, hamster

应用

WB, IHC, ELISA

别名

BZF1, Idol, MIR, MYLIP, PP5242

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman cerebellum tissue, CHO cells
Positive IHC detected inhuman testis tissue, human brain tissue, human heart tissue, human kidney tissue, human liver tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

15455-1-AP targets MYLIP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, hamster samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat, hamster
文献引用反应性human, mouse
免疫原 MYLIP fusion protein Ag7720 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myosin regulatory light chain interacting protein
别名 BZF1, Idol, MIR, MYLIP, PP5242
计算分子量 50 kDa
观测分子量 46-50 kDa
GenBank蛋白编号BC002860
基因名称 MYLIP
Gene ID (NCBI) 29116
RRIDAB_2235610
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WY64
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MYLIP antibody 15455-1-APDownload protocol
IHC protocol for MYLIP antibody 15455-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Elife

SEC24A deficiency lowers plasma cholesterol through reduced PCSK9 secretion.

Authors - Chen Xiao-Wei XW
View Article
mouseWB

Lett Appl Microbiol

Xinnaokang improves cecal microbiota and lipid metabolism to target atherosclerosis.

Authors - R Yang
View Article
humanWB

Heliyon

Identification of ubiquitin markers for survival and prognosis of ovarian cancer

Authors - Yiwen Feng
View Article