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MYLIP Polyclonal antibody

MYLIP Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, hamster

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 15455-1-AP

Print datasheet

Synonyms

BZF1, Idol, MIR, MYLIP, PP5242

验证数据展示

  • WB analysis of human cerebellum using 15455-1-AP

    WB analysis of human cerebellum using 15455-1-AP

    human cerebellum tissue were subjected to SDS PAGE followed by western blot with 15455-1-AP (MYLIP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 15455-1-AP

    IHC staining of human testis using 15455-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 15455-1-AP

    IHC staining of human testis using 15455-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inhuman cerebellum tissue, CHO cells
Positive IHC detected inhuman testis tissue, human brain tissue, human heart tissue, human kidney tissue, human liver tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

15455-1-AP targets MYLIP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, hamster samples.

Tested Applications IHC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat, hamster
Cited Reactivitymouse
Immunogen MYLIP fusion protein Ag7720 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myosin regulatory light chain interacting protein
Synonyms BZF1, Idol, MIR, MYLIP, PP5242
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 46-50 kDa
GenBank Accession NumberBC002860
Gene Symbol MYLIP
Gene ID (NCBI) 29116
RRIDAB_2235610
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WY64
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MYLIP antibody 15455-1-APDownload protocol
IHC protocol for MYLIP antibody 15455-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Elife

SEC24A deficiency lowers plasma cholesterol through reduced PCSK9 secretion.

Authors - Chen Xiao-Wei XW
View Article
mouseWB

Lett Appl Microbiol

Xinnaokang improves cecal microbiota and lipid metabolism to target atherosclerosis.

Authors - R Yang
View Article