验证数据展示
经过测试的应用
Positive WB detected in | HuH-7 cells, mouse testis tissue |
Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:3000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
16963-1-AP targets MYL6B in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, ELISA Application Description |
经测试反应性 | human, mouse |
免疫原 | MYL6B fusion protein Ag10683 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | myosin, light chain 6B, alkali, smooth muscle and non-muscle |
别名 | MLC1SA, MYL6B, Myosin light chain 6B |
计算分子量 | 208 aa, 23 kDa |
观测分子量 | 22-25 kDa |
GenBank蛋白编号 | BC012425 |
基因名称 | MYL6B |
Gene ID (NCBI) | 140465 |
RRID | AB_3085512 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P14649 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MYL6B, also known as MLC1SA, is primarily found in a hexamer consisting of four light chains and two heavy chains. MYL6B is an essential light chain for non-muscle Myosin II (NMII) that is involved in the control of cell adhesion, cell migration and tissue architecture, cargo transport, and endocytosis (PMID: 29439719, 33817240).
实验方案
Product Specific Protocols | |
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WB protocol for MYL6B antibody 16963-1-AP | Download protocol |
IHC protocol for MYL6B antibody 16963-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |