验证数据展示
经过测试的应用
| Positive WB detected in | mouse skeletal muscle tissue, mouse heart tissue, rat skeletal muscle tissues |
| Positive IHC detected in | human colon tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
| IF | See 1 publications below |
产品信息
10324-1-AP targets MYL12B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | MYL12B fusion protein Ag0398 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | myosin, light chain 12B, regulatory |
| 别名 | SHUJUN-1, SHUJUN 1, Myosin regulatory light chain 2-B, smooth muscle isoform, MYLC2B, MRLC2 |
| 计算分子量 | 20 kDa |
| 观测分子量 | 18-20 kDa |
| GenBank蛋白编号 | BC004994 |
| 基因名称 | MYL12B |
| Gene ID (NCBI) | 103910 |
| RRID | AB_2166830 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O14950 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MYL12B is a regulatory subunit of myosin and plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. There are two groups of residues on the MYL12B that are phosphorylated by distinct kinases and have contrasting effects on myosin II biophysical properties.Phosphorylation at Thr18/Ser19 essentially "activates" the myosin molecule to produce force. The second group of phosphorylated residues is at the N-terminus of the MYL12B at Ser1, Ser2 and Thr9 and causes inhibitory effect on myosin activity.(PMID: 22136066)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MYL12B antibody 10324-1-AP | Download protocol |
| IHC protocol for MYL12B antibody 10324-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Biol Cell Multiple regulatory steps control mammalian nonmuscle myosin II assembly in live cells. | ||
Biochemistry (Mosc) Western Blotting-Based Quantitative Measurement of Myosin II Regulatory Light Chain Phosphorylation in Small Amounts of Non-muscle Cells. | ||
BMC Cell Biol Analysis of the role of Ser1/Ser2/Thr9 phosphorylation on myosin II assembly and function in live cells. | ||
J Pept Sci Design of peptidase-resistant peptide inhibitors of myosin light chain kinase. | ||
Biochemistry (Mosc) Long-Term Experimental Hyperglycemia Does Not Impair Macrovascular Endothelial Barrier Integrity and Function in vitro | ||
BMC Microbiol Effects of Saccharomyces boulardii on microbiota composition and metabolite levels in the small intestine of constipated mice |
