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验证数据展示

  • WB analysis using 81204-1-RR (same clone as 81204-1-PBS)

    WB analysis using 81204-1-RR (same clone as 81204-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81204-1-RR (MYH9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81204-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 81204-1-RR (same clone as 81204-1-PBS)

    IHC staining of human colon cancer using 81204-1-RR (same clone as 81204-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81204-1-RR (MYH9 antibody) at dilution of 1:9900 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81204-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYH9 antibody included
  • IHC staining of rat kidney using 81204-1-RR (same clone as 81204-1-PBS)

    IHC staining of rat kidney using 81204-1-RR (same clone as 81204-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 81204-1-RR (MYH9 antibody) at dilution of 1:9900 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81204-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYH9 antibody included
  • IF Staining of HepG2 using 81204-1-RR (same clone as 81204-1-PBS)

    IF Staining of HepG2 using 81204-1-RR (same clone as 81204-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MYH9 antibody (81204-1-RR, Clone: 3E1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81204-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81204-1-RR

    Affinity and Kinetic Characterization of 81204-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81204-1-RR against Human MYH9 were performed. The affinity constant is 0.297 nM.

MYH9 Recombinant monoclonal antibody, PBS Only

MYH9 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 81204-1-PBS

产品说明书

CloneNo. 3E1

宿主/亚型

Rabbit / IgG

种属反应性

rat, mouse, human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

NMMHC II-a, Myosin-9, Myosin heavy chain, non-muscle IIa, Myosin heavy chain 9, Myosin 9

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy MYH9 Ready-To-Use IHC Kit. MYH9 primary antibody included.

产品信息

81204-1-PBS targets MYH9 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with rat, mouse, human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 rat, mouse, human
免疫原

CatNo: Ag1612

Product name: Recombinant human MYH9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1741-1960 aa of BC011915

Sequence: EQGNTELINDRLKKANLQIDQINTDLNLERSHAQKNENARQQLERQNKELKVKLQEMEGTVKSKYKASITALEAKIAQLEEQLDNETKERQAACKQVRRTEKKLKDVLLQVDDERRNAEQYKDQADKASTRLKQLKRQLEEAEEEAQRANASRRKLQRELEDATETADAMNREVSSLKNKLRRGDLPFVVPRRMARKGAGDGSDEEVDGKADGAEAKPAE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 myosin, heavy chain 9, non-muscle
别名 NMMHC II-a, Myosin-9, Myosin heavy chain, non-muscle IIa, Myosin heavy chain 9, Myosin 9
计算分子量 227 kDa
观测分子量224-227 kDa
GenBank蛋白编号BC011915
基因名称 MYH9
Gene ID (NCBI) 4627
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP35579
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MYH9 (Myosin-9) appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. MYH9 is a hexameric protein that consists of two heavy chains (~200 kDa) and two pairs of non-identical light chains (15-22 kDa), a pair of essential or alkali light chains, and a pair of regulatory light chains (PMID: 12800156).

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