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验证数据展示

  • WB analysis using 22280-1-AP (same clone as 22280-1-PBS)

    WB analysis using 22280-1-AP (same clone as 22280-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22280-1-AP (MYH7-specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22280-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 22280-1-AP (same clone as 22280-1-PBS)

    IF Staining of mouse heart using 22280-1-AP (same clone as 22280-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using MYH7-specific antibody (22280-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22280-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 22280-1-AP (same clone as 22280-1-PBS)

    IF Staining of mouse heart using 22280-1-AP (same clone as 22280-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using MYH7-specific antibody (22280-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22280-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse heart using 22280-1-AP (same clone as 22280-1-PBS)

    IP experiment of mouse heart using 22280-1-AP (same clone as 22280-1-PBS)

    IP result of anti-MYH7-specific (IP:22280-1-AP, 4ug; Detection:22280-1-AP 1:3000) with mouse heart tissue lysate 1840 ug. This data was developed using the same antibody clone with 22280-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 22280-1-AP (same clone as 22280-1-PBS)

    IHC staining of mouse skeletal muscle using 22280-1-AP (same clone as 22280-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 22280-1-AP (MYH7-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22280-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYH7 antibody included
  • IHC staining of mouse skeletal muscle using 22280-1-AP (same clone as 22280-1-PBS)

    IHC staining of mouse skeletal muscle using 22280-1-AP (same clone as 22280-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 22280-1-AP (MYH7-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22280-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYH7 antibody included
  • IHC staining of mouse heart using 22280-1-AP (same clone as 22280-1-PBS)

    IHC staining of mouse heart using 22280-1-AP (same clone as 22280-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22280-1-AP (MYH7-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22280-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYH7 antibody included
  • IHC staining of mouse heart using 22280-1-AP (same clone as 22280-1-PBS)

    IHC staining of mouse heart using 22280-1-AP (same clone as 22280-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22280-1-AP (MYH7-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22280-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYH7 antibody included
  • IF Staining of H9C2 using 22280-1-AP (same clone as 22280-1-PBS)

    IF Staining of H9C2 using 22280-1-AP (same clone as 22280-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed H9C2 cells using MYH7-specific antibody (22280-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 22280-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

MYH7-specific Polyclonal antibody, PBS Only

MYH7-specific Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA
Cat No. 22280-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

别名

MYH7, Myosin 7, MYHCB, MyHC-beta, MyHC-slow

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy MYH7 Ready-To-Use IHC Kit. MYH7 primary antibody included.

产品信息

22280-1-PBS targets MYH7-specific in WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myosin, heavy chain 7, cardiac muscle, beta
别名 MYH7, Myosin 7, MYHCB, MyHC-beta, MyHC-slow
计算分子量 223 kDa
观测分子量 200-220 kDa
GenBank蛋白编号NM_000257
基因名称 MYH7
Gene ID (NCBI) 4625
RRIDAB_2736821
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP12883
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Myosin is the major contractile protein of all eukaryotic cells, existing as a hexameric molecule composed of two myosin heavy chains (MHCs) that contain the catalytic sites for ATP hydrolysis, and four lower molecular weight myosin light chains. MYH7 encodes the slow/beta-cardiac myosin heavy chain (MHC), which is the major MHC isoform of cardiac ventricles. It is also expressed in slow, oxidative, type 1 muscle fibers of skeletal muscle. Numerous mutations of MYH7 have been linked to familial hypertrophic cardiomyopathy. This antibody can specifically recognize endogenous MYH7, not cross-reacting with other MHCs (PMID: 2523412, 14520662). Due to the production of Heavy Meromyosin (HMM) during proteolysis of myosin, a band around 150 kDa may be detected, which may correspond to the HMM part of MYH7 (PMID: 14613868).

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