验证数据展示
经过测试的应用
| Positive WB detected in | mouse skeletal muscle tissue, human skeletal muscle tissue, rat skeletal muscle tissue |
| Positive IHC detected in | human skeletal muscle tissue, mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse skeletal muscle tissue |
Planning an IHC experiment? We recommend our IHCeasy MYH4 Ready-To-Use IHC Kit. MYH4 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20140-1-AP targets MYH4-Specific in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig, canine |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | myosin, heavy chain 4, skeletal muscle |
| 别名 | MYH2B, MYH4, MYH4-Specific, MyHC 2B, MyHC IIb, Myosin 4, Myosin heavy chain 2b, Myosin heavy chain 4, Myosin heavy chain IIb |
| 计算分子量 | 223 kDa |
| 观测分子量 | 230 kDa |
| GenBank蛋白编号 | NM_017533 |
| 基因名称 | MYH4 |
| Gene ID (NCBI) | 4622 |
| RRID | AB_10666434 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y623 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Myosin is one of the most important skeletal muscle proteins. MYH4 functions muscle contraction. The antibody is specific to MYH4.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MYH4-Specific antibody 20140-1-AP | Download protocol |
| IHC protocol for MYH4-Specific antibody 20140-1-AP | Download protocol |
| IF protocol for MYH4-Specific antibody 20140-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Adv Bioactive fiber-reinforced hydrogel to tailor cell microenvironment for structural and functional regeneration of myotendinous junction | ||
Nat Commun The nuclear envelope protein Net39 is essential for muscle nuclear integrity and chromatin organization. | ||
Development The long noncoding RNA Meg3 regulates myoblast plasticity and muscle regeneration through epithelial-mesenchymal transition | ||
Am J Pathol Klotho null mutation involvement in adenosine A2B receptor-related skeletal muscle degeneration | ||
Development The long noncoding RNA Meg3 regulates myoblast plasticity and muscle regeneration through epithelial-mesenchymal transition. | ||
Int J Mol Sci Loss of Myostatin Alters Mitochondrial Oxidative Phosphorylation, TCA Cycle Activity, and ATP Production in Skeletal Muscle |
