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验证数据展示

  • WB analysis using 22286-1-AP

    WB analysis using 22286-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22286-1-AP (MYH13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 22286-1-AP

    IHC staining of mouse skeletal muscle using 22286-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 22286-1-AP (MYH13 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYH13 antibody included

MYH13 Polyclonal antibody

MYH13 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22286-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, ELISA

别名

MYH13, MyHC eo, Myosin 13, Myosin heavy chain 13

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse eye tissue, rat eye tissue
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MYH13 Ready-To-Use IHC Kit. MYH13 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22286-1-AP targets MYH13 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myosin, heavy chain 13, skeletal muscle
别名 MYH13, MyHC eo, Myosin 13, Myosin heavy chain 13
计算分子量 224 kDa
观测分子量230-240 kDa
GenBank蛋白编号NM_003802
基因名称 MYH13
Gene ID (NCBI) 8735
RRIDAB_3085693
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UKX3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MYH13 antibody 22286-1-APDownload protocol
IHC protocol for MYH13 antibody 22286-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols