验证数据展示
经过测试的应用
| Positive WB detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | C2C12 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
| ChIP | See 1 publications below |
产品信息
11754-1-AP targets MYF6 in WB, IHC, IF/ICC, chip, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, chip |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, chicken |
| 免疫原 |
CatNo: Ag2350 Product name: Recombinant human MYF6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-242 aa of BC017834 Sequence: MMMDLFETGSYFFYLDGENVTLQPLEVAEGSPLYPGSDGTLSPCQDQMPPEAGSDSSGEEHVLAPPGLQPPHCPGQCLIWACKTCKRKSAPTDRRKAATLRERRRLKKINEAFEALKRRTVANPNQRLPKVEILRSAISYIERLQDLLHRLDQQEKMQELGVDPFSYRPKQENLEGADFLRTCSSQWPSVSDHSRGLVITAKEGGASIDSSASSSLRCLSSIVDSISSEERKLPCVEEVVEK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | myogenic factor 6 (herculin) |
| 别名 | Myf-6, Myf 6, Muscle-specific regulatory factor 4, MRF4, Class C basic helix-loop-helix protein 4 |
| 计算分子量 | 242 aa, 27 kDa |
| 观测分子量 | 27 kDa |
| GenBank蛋白编号 | BC017834 |
| 基因名称 | MYF6 |
| Gene ID (NCBI) | 4618 |
| RRID | AB_2250929 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P23409 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for MYF6 antibody 11754-1-AP | Download protocol |
| IHC protocol for MYF6 antibody 11754-1-AP | Download protocol |
| WB protocol for MYF6 antibody 11754-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun MRF4 negatively regulates adult skeletal muscle growth by repressing MEF2 activity. | ||
Front Cell Dev Biol A Functional SNP in the Promoter of LBX1 Is Associated With the Development of Adolescent Idiopathic Scoliosis Through Involvement in the Myogenesis of Paraspinal Muscles. | ||
In Vitro Cell Dev Biol Anim Expression patterns and correlation analyses of muscle-specific genes in the process of sheep myoblast differentiation | ||
Heliyon Exogenous hydrogen sulfide enhances myogenic differentiation of C2C12 myoblasts under high palmitate stress | ||
Stem Cells Int Noggin Combined With Human Dental Pulp Stem Cells to Promote Skeletal Muscle Regeneration | ||
Poult Sci Dysfunctional terminal differentiation of satellite cells in the wooden breast muscle of Ross 308 broiler chickens |
