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验证数据展示

  • IHC staining of human brain using 27951-1-AP

    IHC staining of human brain using 27951-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27951-1-AP (MYCBP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYCBP2 antibody included
  • IHC staining of human brain using 27951-1-AP

    IHC staining of human brain using 27951-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27951-1-AP (MYCBP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYCBP2 antibody included
  • IF Staining of U2OS using 27951-1-AP

    IF Staining of U2OS using 27951-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MYCBP2 antibody (27951-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • FC experiment of U2OS using 27951-1-AP

    FC experiment of U2OS using 27951-1-AP

    1x10^6 U2OS cells were intracellularly stained with 0.4 ug MYCBP2 Polyclonal antibody (27951-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

MYCBP2 Polyclonal antibody

MYCBP2 Polyclonal Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 27951-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF/ICC, FC (Intra), ELISA

别名

KIAA0916, MYC binding protein 2, PAM, Pam/highwire/rpm 1 protein, Protein associated with Myc

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman brain tissue, rat brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy MYCBP2 Ready-To-Use IHC Kit. MYCBP2 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 2 publications below

产品信息

27951-1-AP targets MYCBP2 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF/ICC, FC (Intra), ELISA Application Description
文献引用应用IHC
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag27228

Product name: Recombinant human MYCBP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 2672-2880 aa of NM_015057

Sequence: MGNSKGDRGNISTSSKPASTSGKSELSSKHSRSLKPDGRMSRTTADQKKPRGTESLSASESLILKSDAAKLRSDSHSRSLSPNHNTLQTLKSDGRMPSSSRAESPGPGSRLSSPKPKTLPANRSSPSGASSPRSSSPHDKNLPQKSTAPVKTKLDPPRERSKSDSYTLDPDTLRKKKMPLTEPLRGRSTSPKPKSVPKDSTDSPGSENRA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 MYC binding protein 2
别名 KIAA0916, MYC binding protein 2, PAM, Pam/highwire/rpm 1 protein, Protein associated with Myc
计算分子量 510 kDa
GenBank蛋白编号NM_015057
基因名称 MYCBP2
Gene ID (NCBI) 23077
RRIDAB_2881020
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75592
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for MYCBP2 antibody 27951-1-APDownload protocol
IF protocol for MYCBP2 antibody 27951-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Immunol

MYCBP2 expression correlated with inflammatory cell infiltration and prognosis immunotherapy in thyroid cancer patients

Authors - Guilin Wang
View Article
humanIHC

Arch Biochem Biophys

Exosomes from human umbilical cord mesenchymal stem cells protect aortas in Db/db mice characterized by combination of metabolomics and proteomics

Authors - Ming-Ying Ling
View Article