验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, A549 cells, HEK-293 cells, Jurkat cells |
Planning an IHC experiment? We recommend our IHCeasy MYCBP Ready-To-Use IHC Kit. MYCBP primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85891-1-RR targets MYCBP in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | MYCBP fusion protein Ag2651 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | c-myc binding protein |
| 别名 | AMY 1, AMY1, AMY-1, Associate of Myc 1, c-Myc-binding protein |
| 计算分子量 | 103 aa, 12 kDa |
| 观测分子量 | 12 kDa |
| GenBank蛋白编号 | BC008686 |
| 基因名称 | MYCBP |
| Gene ID (NCBI) | 26292 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q99417 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MYC binding protein(MYCBP), encoded by MYCBP gene, binds to N-terminal region of MYC, and then MYC can activate E box-dependent transcription. Upon increasing expression of MYC , MYCBP translocates into the nucleus in the S phase of the cell cycle. MYCBP may involve in spermatogenesis, since it's found to be interacted with AKAP84/149 in the mitochondria in somatic cells and sperm. It highly expressed in heart, placenta, pancreas,skeletal muscle and kidney, but low in lung. This is a rabbit polyclonal antibody raised against full-length MYCBP of human origin.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MYCBP antibody 85891-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |