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验证数据展示

  • WB analysis of recombinant proein using 60003-2-Ig (same clone as 60003-2-PBS)

    WB analysis of recombinant proein using 60003-2-Ig (same clone as 60003-2-PBS)

    Western blot of Myc-tagged fusion protein with anti-Myc-tag (60003-2-Ig) at various dilutions. This data was developed using the same antibody clone with 60003-2-PBS in a different storage buffer formulation.

  • IF Staining of Transfected HEK-293 using 60003-2-Ig (same clone as 60003-2-PBS)

    IF Staining of Transfected HEK-293 using 60003-2-Ig (same clone as 60003-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using MYC tag antibody (60003-2-Ig, Clone: 1A5A2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60003-2-PBS in a different storage buffer formulation.

  • IP experiment of Transfected HEK-293 using 60003-2-Ig (same clone as 60003-2-PBS)

    IP experiment of Transfected HEK-293 using 60003-2-Ig (same clone as 60003-2-PBS)

    IP result of anti-MYC tag (IP:60003-2-Ig, 4ug; Detection:60003-2-Ig 1:10000) with Transfected HEK-293 cells lysate 300ug. This data was developed using the same antibody clone with 60003-2-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 transfected with GFP-MYC using 60003-2-Ig (same clone as 60003-2-PBS)

    FC experiment of HEK-293 transfected with GFP-MYC using 60003-2-Ig (same clone as 60003-2-PBS)

    1x10^6 HEK-293 transfected with GFP-MYC were intracellularly stained with 0.25 ug MYC tag Monoclonal antibody (60003-2-Ig, Clone:1A5A2) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(RGAM005). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60003-2-PBS in a different storage buffer formulation.

MYC tag Monoclonal antibody, PBS Only

MYC tag Monoclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA
Cat No. 60003-2-PBS

产品说明书

CloneNo. 1A5A2

宿主/亚型

Mouse / IgG1

种属反应性

recombinant protein

应用

WB, IF/ICC, FC (Intra), IP, ELISA

别名

myc, EQKLISEEDL

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

60003-2-PBS targets MYC tag in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with recombinant protein samples.

经测试应用 WB, IF/ICC, FC (Intra), IP, ELISA Application Description
经测试反应性 recombinant protein
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 Myc tag
别名 myc, EQKLISEEDL
基因名称 Myc tag
Gene ID (NCBI) 99
RRIDAB_2734122
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The c-Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.