MYBPC3 Polyclonal antibody
MYBPC3 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, rat heart tissue, human heart tissue |
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse heart tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IHC | See 1 publications below |
产品信息
19977-1-AP targets MYBPC3 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC, IHC, WB, ELISA |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | myosin binding protein C, cardiac |
| Synonyms | Cardiac MyBP C, CMH4, FHC, MYBP C, MYBPC3 |
| Calculated Molecular Weight | 141 kDa |
| Observed Molecular Weight | 141-150 kDa |
| GenBank Accession Number | NM_000256 |
| Gene Symbol | MYBPC3 |
| Gene ID (NCBI) | 4607 |
| RRID | AB_10858232 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14896 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MYBPC3 belongs to the immunoglobulin superfamily and MyBP family. MYBPC3 located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role. Defects in MYBPC3 are the cause of cardiomyopathy familial hypertrophic type 4 (CMH4). The antibody detects the full length form and the degradation bands around 28-54 kDa in SDS-PAGE.(PMID: 22076249)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MYBPC3 antibody 19977-1-AP | Download protocol |
| IHC protocol for MYBPC3 antibody 19977-1-AP | Download protocol |
| IF protocol for MYBPC3 antibody 19977-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
FASEB J Amplified pathogenic actions of angiotensin II in cysteine-rich LIM-only protein 4-negative mouse hearts. | ||
Front Cardiovasc Med Case Report: Identification of the First Synonymous Variant of Myosin Binding Protein C3 (c.24A>C, p.P8P) Altering RNA Splicing in a Cardiomyopathy and Sudden Cardiac Death Case. | ||
Front Cardiovasc Med Hypercontractile cardiac phenotype in mice overexpressing the regulatory subunit PR72 of protein phosphatase 2A |
