验证数据展示
经过测试的应用
| Positive WB detected in | mouse skeletal muscle tissue, mouse pancreas tissue |
| Positive IHC detected in | human skeletal muscle tissue, human bladder tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IHC | See 2 publications below |
产品信息
22900-1-AP targets MYBPC1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | myosin binding protein C, slow type |
| 别名 | MYBPC1, MYBPCC, MYBPCS, Slow MyBP C, slow type |
| 计算分子量 | 128 kDa |
| 观测分子量 | 135-140 kDa |
| GenBank蛋白编号 | NM_002465 |
| 基因名称 | MYBPC1 |
| Gene ID (NCBI) | 4604 |
| RRID | AB_11182592 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q00872 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MYBPC1, also named as MYBPCS, belongs to the immunoglobulin superfamily and MyBP family. It is a thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role. This antibody is specific to MYBPC1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MYBPC1 antibody 22900-1-AP | Download protocol |
| IHC protocol for MYBPC1 antibody 22900-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Psychiatry FXR1 regulation of parvalbumin interneurons in the prefrontal cortex is critical for schizophrenia-like behaviors. | ||
Dokl Biochem Biophys Early Deсline in Rat Soleus Passive Tension with Hindlimb Unloading: Inactivation of Cross-bridges or Activation of Calpains? | ||
Adv Biol (Weinh) Glucose-Responsive Fibrin Hydrogel-Based Multimodal Nucleic Acid Delivery System |
