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验证数据展示

  • WB analysis using 67996-1-Ig (same clone as 67996-1-PBS)

    WB analysis using 67996-1-Ig (same clone as 67996-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67996-1-Ig (MYBBP1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

  • WB analysis of HSC-T6 using 67996-1-Ig (same clone as 67996-1-PBS)

    WB analysis of HSC-T6 using 67996-1-Ig (same clone as 67996-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67996-1-Ig (MYBBP1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 67996-1-Ig (same clone as 67996-1-PBS)

    WB analysis of HEK-293 using 67996-1-Ig (same clone as 67996-1-PBS)

    WB result of MYBBP1A antibody (67996-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYBBP1A transfected HEK-293 cells. This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 67996-1-Ig (same clone as 67996-1-PBS)

    IHC staining of human kidney using 67996-1-Ig (same clone as 67996-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67996-1-Ig (MYBBP1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYBBP1A antibody included
  • IF Staining of HepG2 using 67996-1-Ig (same clone as 67996-1-PBS)

    IF Staining of HepG2 using 67996-1-Ig (same clone as 67996-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MYBBP1A antibody (67996-1-Ig, Clone: 1H2D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67996-1-Ig (same clone as 67996-1-PBS)

    IF Staining of HepG2 using 67996-1-Ig (same clone as 67996-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MYBBP1A antibody (67996-1-Ig, Clone: 1H2D10 ) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 67996-1-Ig (same clone as 67996-1-PBS)

    IF Staining of NIH/3T3 using 67996-1-Ig (same clone as 67996-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using MYBBP1A antibody (67996-1-Ig, Clone: 1H2D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 67996-1-Ig (same clone as 67996-1-PBS)

    IF Staining of NIH/3T3 using 67996-1-Ig (same clone as 67996-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using MYBBP1A antibody (67996-1-Ig, Clone: 1H2D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

MYBBP1A Monoclonal antibody, PBS Only

MYBBP1A Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67996-1-PBS

产品说明书

CloneNo. 1H2D10

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

1H2D10, Myb binding protein 1A, Myb-binding protein 1A, PAP2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy MYBBP1A Ready-To-Use IHC Kit. MYBBP1A primary antibody included.

产品信息

67996-1-PBS targets MYBBP1A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6008

Product name: Recombinant human MYBBP1A protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 979-1328 aa of BC050546

Sequence: STALSSFLTKRNSPLTVPMFLSLFSRHPVLCQSLLPILVQHITGPVRPRRQACLLLQKTLSMREVRSCFEDPEWKQLMGQVLAKVTENLRVLGEAQTKAQHQQALSSLELLNVLFRTCKHEKLTLDLTVLLGVLQGQQQSLQQGAHSTGSSRLHDLYWQAMKTLGVQRPKLEKKDAKEIPSATQSPISKKRKKKGFLPETKKRKKRKSEDGTPAEDGTPAATGGSQPPSMGRKKRNRTKAKVPAQANGTPTTKSPAPGAPTRSPSTPAKSPKLQKKNQKPSQVNGAPGSPTEPAGQKQHQKALPKKGVLGKSPLSALARKKARLSLVIRSPSLLQSGAKKKAQVRKAGKP

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 MYB binding protein (P160) 1a
别名 1H2D10, Myb binding protein 1A, Myb-binding protein 1A, PAP2
计算分子量 149 kDa
观测分子量140 kDa
GenBank蛋白编号BC050546
基因名称 MYBBP1A
Gene ID (NCBI) 10514
RRIDAB_2918745
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9BQG0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The protooncogene MYB is predominantly expressed in immature hemopoietic cells where it has an essential role in hemopoietic cell proliferation and differentiation. Oncogenically activated forms of MYB is generally N- and/or C-terminal truncations of the normal MYB protein. Removal of the C terminus of MYB disrupts or deletes a region termed the negative regulatory domain (NRD), resulting in an increase in DNA binding, transactivation, and transformation by MYB. One feature of the NRD is a leucine zipper-like motif [PMID: 8302594]. Murine Myb-binding protein-1a (MYBBP1A), originally called P160, was identified by its ability to interact specifically with Myb via this leucine zipper-like motif. MYBBP1A modulates MYB activity upon binding to the MYB NRD [PMID: 10644447, 9447996].

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