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MXD1 Polyclonal antibody

MXD1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 19547-1-AP

Print datasheet

Synonyms

MAD, MAD1, MAX dimerization protein 1, Max dimerizer 1, MXD1, Protein MAD

验证数据展示

  • WB analysis of HepG2 using 19547-1-AP

    WB analysis of HepG2 using 19547-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 19547-1-AP (MXD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 19547-1-AP

    IP experiment of HepG2 using 19547-1-AP

    IP result of anti-MXD1 (IP:19547-1-AP, 4ug; Detection:19547-1-AP 1:500) with HepG2 cells lysate 3000ug.

经过测试的应用

Positive WB detected inHepG2 cells
Positive IP detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

ChIPSee 1 publications below

产品信息

19547-1-AP targets MXD1 in WB, IP, chIP, ELISA applications and shows reactivity with human samples.

Tested Applications IP, WB, ELISA
Cited ApplicationschIP
Tested Reactivity human
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name MAX dimerization protein 1
Synonyms MAD, MAD1, MAX dimerization protein 1, Max dimerizer 1, MXD1, Protein MAD
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberNM_002357
Gene Symbol MXD1
Gene ID (NCBI) 4084
RRIDAB_10641995
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ05195
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MXD1, also named as MAD, is a transcriptional repressor. MAD1 binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. MAD thus antagonizes MYC transcriptional activity by competing for MAX. There're two isoforms of MXD1 protein.

实验方案

Product Specific Protocols
WB protocol for MXD1 antibody 19547-1-APDownload protocol
IP protocol for MXD1 antibody 19547-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanchIP

J Biol Chem

Role for carbohydrate response element-binding protein (ChREBP) in high glucose-mediated repression of long noncoding RNA Tug1.

Authors - Jianyin Long
View Article