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验证数据展示

  • WB analysis of HepG2 using 19547-1-AP

    WB analysis of HepG2 using 19547-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 19547-1-AP (MXD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 19547-1-AP

    IP experiment of HepG2 using 19547-1-AP

    IP result of anti-MXD1 (IP:19547-1-AP, 4ug; Detection:19547-1-AP 1:800) with HepG2 cells lysate 2000 ug.

MXD1 Polyclonal antibody

MXD1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 19547-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, ELISA and More (1)

别名

MAD, MAD1, MAX dimerization protein 1, Max dimerizer 1, Protein MAD

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells
Positive IP detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19547-1-AP targets MXD1 in WB, IP, chIP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB, chIP
经测试反应性 human
文献引用反应性human
免疫原

fusion protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 MAX dimerization protein 1
别名 MAD, MAD1, MAX dimerization protein 1, Max dimerizer 1, Protein MAD
计算分子量 25 kDa
观测分子量 25 kDa
GenBank蛋白编号NM_002357
基因名称 MXD1
Gene ID (NCBI) 4084
RRIDAB_10641995
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ05195
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MXD1, also named as MAD, is a transcriptional repressor. MAD1 binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. MAD thus antagonizes MYC transcriptional activity by competing for MAX. There're two isoforms of MXD1 protein.

实验方案

Product Specific Protocols
WB protocol for MXD1 antibody 19547-1-APDownload protocol
IP protocol for MXD1 antibody 19547-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanchIP

J Biol Chem

Role for carbohydrate response element-binding protein (ChREBP) in high glucose-mediated repression of long noncoding RNA Tug1.

Authors - Jianyin Long
View Article
humanWB,ChIP

Theranostics

BARD1-mediated stabilization of METTL14 promotes retinal neovascularization by m6A-modifying MXD1 mRNA on a YTHDF2-dependent manner

Authors - Xianyang Liu
View Article