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验证数据展示

  • WB analysis of HepG2 using 15331-1-PBS

    WB analysis of HepG2 using 15331-1-PBS

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15331-1-AP (MVD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis using 15331-1-AP (same clone as 15331-1-PBS)

    WB analysis using 15331-1-AP (same clone as 15331-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15331-1-AP (MVD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15331-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 15331-1-AP (same clone as 15331-1-PBS)

    IHC staining of human lung cancer using 15331-1-AP (same clone as 15331-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15331-1-AP (MVD Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15331-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 15331-1-AP (same clone as 15331-1-PBS)

    IHC staining of human lung cancer using 15331-1-AP (same clone as 15331-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15331-1-AP (MVD Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15331-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 15331-1-AP (same clone as 15331-1-PBS)

    IHC staining of human colon using 15331-1-AP (same clone as 15331-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15331-1-AP (MVD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15331-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 15331-1-AP (same clone as 15331-1-PBS)

    IF Staining of A431 using 15331-1-AP (same clone as 15331-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using MVD antibody (15331-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 15331-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 15331-1-AP (same clone as 15331-1-PBS)

    IF Staining of A549 using 15331-1-AP (same clone as 15331-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using MVD antibody (15331-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 15331-1-PBS in a different storage buffer formulation.

MVD Polyclonal antibody, PBS Only

MVD Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15331-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

diphospho, EC:4.1.1.33, Mevalonate, MPD

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

15331-1-PBS targets MVD in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7255

Product name: Recombinant human MVD protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-400 aa of BC000011

Sequence: MASEKPLAAVTCTAPVNIAVIKYWGKRDEELVLPINSSLSVTLHQDQLKTTTTAVISKDFTEDRIWLNGREEDVGQPRLQACLREIRCLARKRRNSRDGDPLPSSLSCKVHVASVNNFPTAAGLASSAAGYACLAYTLARVYGVESDLSEVARRGSGSACRSLYGGFVEWQMGEQADGKDSIARQVAPESHWPELRVLILVVSAEKKLTGSTVGMRASVETSPLLRFRAESVVPARMAEMARCIRERDFPSFAQLTMKDSNQFHATCLDTFPPISYLNAISWRIIHLVHRFNAHHGDTKVAYTFDAGPNAVIFTLDDTVAEFVAAVWHGFPPGSNGDTFLKGLQVRPAPLSAELQAALAMEPTPGGVKYIIVTQVGPGPQILDDPCAHLLGPDGLPKPAA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mevalonate (diphospho) decarboxylase
别名 diphospho, EC:4.1.1.33, Mevalonate, MPD
计算分子量 43 kDa
观测分子量 66-74 kDa, 45 kDa, 37 kDa
GenBank蛋白编号BC000011
基因名称 MVD
Gene ID (NCBI) 4597
RRIDAB_2147439
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP53602
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The enzyme mevalonate pyrophosphate decarboxylase(MVD) catalyzes the conversion of mevalonate pyrophosphate into isopentenyl pyrophosphate. It is also named as MPD and as a unique enzyme in one of the early steps in cholesterol biosynthesis, MVD may be a useful target for drugs aimed at lowering serum cholesterol levels(PMID:8626466). The intracellular glycosylation does not contribute to the difference between the 45 and 37 kDa species of MVD. The native MVD has a molecular weight of 90 kDa that it consists of two identical subunits of 45 kDa and a 37 kDa protein is also found as a subunit of MVD and this type of MVD may be a 74 kDa.But the 37 kDa enzyme appeared only when the rats are fed the CP diet.(PMID:9348097).

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