验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
33961-1-AP targets MVB12B in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag40577 Product name: Recombinant human FAM125B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 31-200 aa of BC000122 Sequence: TMPEVKDLSEALPETSMDPITGVGVVASRNRAPTGYDVVAQTADGVDADLWKDGLFKSKVTRYLCFTRSFSKENSHLGNVLVDMKLIDIKDTLPVGFIPIQETVDTQEVAFRKKRLCIKFIPRDSTEAAICDIRIMGRTKQAPPQYTFIGELNSMGIWYRMGRVPRNHDS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | family with sequence similarity 125, member B |
| 别名 | C9orf28, ESCRT-I complex subunit MVB12B, FAM125B, Multivesicular body subunit 12B, Protein FAM125B |
| 计算分子量 | 319 aa, 36 kDa |
| 观测分子量 | 35 kDa |
| GenBank蛋白编号 | BC000122 |
| 基因名称 | FAM125B |
| Gene ID (NCBI) | 89853 |
| RRID | AB_3743125 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q9H7P6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FAM125B/MVB12B gene codes for a component of a membrane complex involved in vesicular trafficking process, a function similar to that of the Caveolin genes (CAV1 and CAV2) which have previously been associated with primary open-angle glaucoma (PMID: 24518671). MVB12b is also a target for TANK-binding kinase 1 phosphorylation, which is essential for the sorting of DNA into EVs and stimulation of bystander cells (PMID: 30804548).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MVB12B antibody 33961-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
