验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells |
| Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
产品信息
24015-1-AP targets MUTED/BLOC1S5 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag21226 Product name: Recombinant human MUTED protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-108 aa of BC119644 Sequence: MSGGGTETPVGCEAAPGGGSKKRDSLGTAGSAHLIIKDLGEIHSRLLDHRPVIQGETRYFVKEFEEKRGLREMRVLENLKNMIHETNEHTLPKCRDTMRDSLSQVLQR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | muted homolog (mouse) |
| 别名 | MUTED, MU, BLOC1S5, BLOC-1 subunit 5, BL1S5 |
| 计算分子量 | 187 aa, 22 kDa |
| 观测分子量 | 22-25 kDa |
| GenBank蛋白编号 | BC119644 |
| 基因名称 | MUTED |
| Gene ID (NCBI) | 63915 |
| RRID | AB_2879402 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8TDH9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BLOC1S5 (biogenesis of lysosomal organelles complex 1 subunit 5) encodes a subunit of the BLOC1, a complex that is involved in transport of some but not all cargo to the lysosome and lysosome-related organelles. BLOC1S5 has a crucial role in STING-mediated cell death upon stimulation with low concentrations of CMA(PMID: 29033128).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for MUTED/BLOC1S5 antibody 24015-1-AP | Download protocol |
| WB protocol for MUTED/BLOC1S5 antibody 24015-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell The DNA Inflammasome in Human Myeloid Cells Is Initiated by a STING-Cell Death Program Upstream of NLRP3.
| ||
