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验证数据展示

  • WB analysis of HEK-293T using 24015-1-AP

    WB analysis of HEK-293T using 24015-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 24015-1-AP (MUTED Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 24015-1-AP

    IF Staining of HEK-293 using 24015-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 24015-1-AP (MUTED antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

MUTED/BLOC1S5 Polyclonal antibody

MUTED/BLOC1S5 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 24015-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IF/ICC, ELISA

别名

MUTED, MU, BLOC1S5, BLOC-1 subunit 5, BL1S5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24015-1-AP targets MUTED/BLOC1S5 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human, mouse
免疫原 MUTED/BLOC1S5 fusion protein Ag21226 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 muted homolog (mouse)
别名 MUTED, MU, BLOC1S5, BLOC-1 subunit 5, BL1S5
计算分子量 187 aa, 22 kDa
观测分子量 22-25 kDa
GenBank蛋白编号BC119644
基因名称 MUTED
Gene ID (NCBI) 63915
RRIDAB_2879402
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TDH9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BLOC1S5 (biogenesis of lysosomal organelles complex 1 subunit 5) encodes a subunit of the BLOC1, a complex that is involved in transport of some but not all cargo to the lysosome and lysosome-related organelles. BLOC1S5 has a crucial role in STING-mediated cell death upon stimulation with low concentrations of CMA(PMID: 29033128).

实验方案

Product Specific Protocols
WB protocol for MUTED/BLOC1S5 antibody 24015-1-APDownload protocol
IF protocol for MUTED/BLOC1S5 antibody 24015-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell

The DNA Inflammasome in Human Myeloid Cells Is Initiated by a STING-Cell Death Program Upstream of NLRP3.

Authors - Moritz M Gaidt
  • KO Validated
View Article
mouseWB

Cells

Dysbindin deficiency Alters Cardiac BLOC-1 Complex and Myozap Levels in Mice.

Authors - Ankush Borlepawar
View Article