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CoraLite® Plus 488-conjugated MUM1/IRF4 Polyclonal antibody

MUM1/IRF4 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-11247

Print datasheet

Synonyms

interferon regulatory factor 4, IRF 4, IRF4, LSIRF, Multiple myeloma oncogene 1, MUM1, MUM1/IRF4, NF EM5

验证数据展示

  • FC experiment of Ramos using CL488-11247

    FC experiment of Ramos using CL488-11247

    1X10^6 Ramos cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human MUM1/IRF4 (CL488-11247) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • FC experiment of Ramos using CL488-11247

    FC experiment of Ramos using CL488-11247

    1X10^6 Ramos cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human MUM1/IRF4 (CL488-11247) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC detected inRamos cells
Planning an IHC experiment? We recommend our IHCeasy IRF4 Ready-To-Use IHC Kit. IRF4 primary antibody included.

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11247 targets MUM1/IRF4 in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra)
Tested Reactivity human
Immunogen MUM1/IRF4 fusion protein Ag1762 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name IRF 4
Synonyms interferon regulatory factor 4, IRF 4, IRF4, LSIRF, Multiple myeloma oncogene 1, MUM1, MUM1/IRF4, NF EM5
Calculated Molecular Weight 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC015752
Gene Symbol IRF4
Gene ID (NCBI) 3662
ENSEMBL Gene IDENSG00000137265
RRIDAB_2919019
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15306
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IRF4 is a member of the IRF family of transcription factors, expressed in most cell types of the immune system. IRF4 is a transcription factor essential for the development of T helper-2 (Th2) cells, IL17-producing Th17 cells, and IL9-producing Th9 cells, as well as dendritic cell (DC). It binds to and activates the ISRE of the MHC class I promoter, and may have a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 MUM1/IRF4 antibody CL488-11247Download protocol
Standard Protocols
Click here to view our Standard Protocols