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验证数据展示

  • WB analysis of A549 using 55489-1-AP

    WB analysis of A549 using 55489-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 55489-1-AP (MUC8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 55489-1-AP

    IHC staining of human lung cancer using 55489-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 55489-1-AP (MUC8 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human lung cancer using 55489-1-AP

    IHC staining of human lung cancer using 55489-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 55489-1-AP (MUC8 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of A549 using 55489-1-AP

    IF Staining of A549 using 55489-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using MUC8 antibody (55489-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

MUC8 Polyclonal antibody

MUC8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 55489-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

mucin 8

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells
Positive IHC detected inhuman lung cancer tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55489-1-AP targets MUC8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mucin 8
别名 mucin 8
计算分子量 33 kDa
观测分子量 33-38 kDa
GenBank蛋白编号U14383
基因名称 MUC8
Gene ID (NCBI) 4590
RRIDAB_11182275
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mucin glycoproteins (Mucins) are major constituents of the glycocalyx that covers mucosal epithelium. MUC8 is one of the major mucins in the ethmoid mucosa and is upregulated by chronic inflammation. TNFa, IL-1b and a combination of both can significantly increase Mucin 8 mRNA levels, suggesting that a mixture of inflammatory mediators can synergistically increase secretion of mucin in human nasal epithelium. This antibody is specific to MUC8.

实验方案

Product Specific Protocols
WB protocol for MUC8 antibody 55489-1-APDownload protocol
IHC protocol for MUC8 antibody 55489-1-APDownload protocol
IF protocol for MUC8 antibody 55489-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC,WB

Diagnostics (Basel)

The Translational Role of MUC8 in Salivary Glands: A Potential Biomarker for Salivary Stone Disease?

Authors - Martin Schicht
View Article