验证数据展示
经过测试的应用
| Positive IHC detected in | human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse lung tissue |
Planning an IHC experiment? We recommend our IHCeasy MUC5B Ready-To-Use IHC Kit. MUC5B primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
28118-1-AP targets MUC5B in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat, pig |
| 免疫原 |
CatNo: Ag27886 Product name: Recombinant human MUC5B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 5548-5716 aa of NM_002458 Sequence: MLSGDTQDPTVQCQEDACNNTTCPQGFEYKRVAGQCCGECVQTACLTPDGQPVQLNETWVNSHVDNCTVYLCEAEGGVHLLTPQPASCPDVSSCRGSLRKTGCCYSCEEDSCQVRINTTILWHQGCETEVNITFCEGSCPGASKYSAEAQAMQHQCTCCQERRVHEETVP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | mucin 5B, oligomeric mucus/gel-forming |
| 别名 | MUC9, MUC5, MG1 |
| 计算分子量 | 596 kDa |
| GenBank蛋白编号 | NM_002458 |
| 基因名称 | MUC5B |
| Gene ID (NCBI) | 727897 |
| RRID | AB_2881067 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9HC84 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for MUC5B antibody 28118-1-AP | Download protocol |
| IHC protocol for MUC5B antibody 28118-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Clin Transl Med Single-cell transcriptomes reveal heterogeneity of high-grade serous ovarian carcinoma. | ||
J Ethnopharmacol Synergistic effect of constituent drugs of Baibutang on improving Yin-deficiency pulmonary fibrosis in rats | ||
Front Immunol Establishment of a porcine bronchial epithelial cell line and its application to study innate immunity in the respiratory epithelium | ||
Cells Bleomycin-Induced Pulmonary Fibrosis in Transgenic Mice Carrying the Human MUC5B rs35705950 Variant |
