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MUC20 Polyclonal antibody

MUC20 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 13380-1-AP

Print datasheet

Synonyms

KIAA1359, MUC 20, MUC20, Mucin 20

验证数据展示

  • IP experiment of mouse liver using 13380-1-AP

    IP experiment of mouse liver using 13380-1-AP

    IP result of anti-MUC20 (IP:13380-1-AP, 4ug; Detection:13380-1-AP 1:300) with mouse liver tissue lysate 6000 ug.

  • WB analysis of human liver using 13380-1-AP

    WB analysis of human liver using 13380-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 13380-1-AP (MUC20 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inhuman liver tissue, human kidney tissue
Positive IP detected inmouse liver tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

13380-1-AP targets MUC20 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen MUC20 fusion protein Ag4205 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mucin 20, cell surface associated
Synonyms KIAA1359, MUC 20, MUC20, Mucin 20
Calculated Molecular Weight 55 kDa, 71 kDa
Observed Molecular Weight 70-75 kDa
GenBank Accession NumberBC029267
Gene Symbol MUC20
Gene ID (NCBI) 200958
RRIDAB_2235526
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N307
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MUC20 contains serine, threonine, and proline-rich repeats in its extracellular domain and is likely to be extensively O-glycosylated. MUC20 blocks GRB2 recruitment to MET thus suppressing the GRB2-RAS pathway and inhibiting HGF-induced proliferation of MMP1 and MMP9 expression (PMID: 15314156). MUC20 is highly expressed in the kidney and localized in the proximal tubules but not in the glomerulus or distal tubules(PMID: 14565953). MUC20 was detected in most of the male urogenital tract epithelia, except for epididymis(PMID: 16997931). The expression of MUC20 is up-regulated in the kidneys of patients with immunoglobulin A nephropathy, in an animal model of lupus nephritis, and in mice with acute renal injury caused by cisplatin administration or unilateral ureteral obstruction(PMID: 14565953).

实验方案

Product Specific Protocols
WB protocol for MUC20 antibody 13380-1-APDownload protocol
IP protocol for MUC20 antibody 13380-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Exp Clin Cancer Res

MUC20 regulated by extrachromosomal circular DNA attenuates proteasome inhibitor resistance of multiple myeloma by modulating cuproptosis

Authors - Xiaobin Wang
View Article