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验证数据展示

  • IHC staining of human colon using 82792-2-RR

    IHC staining of human colon using 82792-2-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82792-2-RR (MUC2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MUC2 antibody included
  • IHC staining of human colon using 82792-2-RR

    IHC staining of human colon using 82792-2-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82792-2-RR (MUC2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MUC2 antibody included
  • IHC staining of mouse colon using 82792-2-RR

    IHC staining of mouse colon using 82792-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 82792-2-RR (MUC2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MUC2 antibody included
  • IHC staining of mouse colon using 82792-2-RR

    IHC staining of mouse colon using 82792-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 82792-2-RR (MUC2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MUC2 antibody included
  • IHC staining of human colon using 82792-2-RR

    IHC staining of human colon using 82792-2-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82792-2-RR (MUC2 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MUC2 antibody included
  • IF Staining of human colon using 82792-2-RR

    IF Staining of human colon using 82792-2-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon tissue using MUC2 antibody (82792-2-RR, Clone: 250669F4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse colon using 82792-2-RR

    IF Staining of mouse colon using 82792-2-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using MUC2 antibody (82792-2-RR, Clone: 250669F4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 82792-2-RR

    Affinity and Kinetic Characterization of 82792-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82792-2-RR against Human MUC2 were performed. The affinity constant is below 1 pM.

MUC2 Recombinant monoclonal antibody

MUC2 Uni-rAb® Recombinant Antibody for IHC, IF-P, ELISA
Cat No. 82792-2-RR

产品说明书

CloneNo. 250669F4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF-P, ELISA

别名

MUC 2, MUC-2, Mucin 2, SMUC

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inmouse colon tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse colon tissue, human colon tissue
Planning an IHC experiment? We recommend our IHCeasy MUC2 Ready-To-Use IHC Kit. MUC2 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82792-2-RR targets MUC2 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag25800

Product name: Recombinant human MUC2 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 726-850 aa of M94132

Sequence: MYLEAGDVVVRQEERCVCRDGRLHCRQIRLIGQSCTAPKIHMDCSNLTALATSKPRALSCQTLAAGYYHTECVSGCVCPDGLMDDGRGGCVVEKECPCVHNNDLYSSGAKIKVDCNTCTCKRGRWV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mucin 2, oligomeric mucus/gel-forming
别名 MUC 2, MUC-2, Mucin 2, SMUC
计算分子量 540 kDa
GenBank蛋白编号M94132
基因名称 MUC2
Gene ID (NCBI) 4583
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins produced by many epithelial tissues. The protein encoded by this gene is secreted and forms an insoluble mucous barrier that protects the gut lumen. The protein polymerizes into a gel of which 80% is composed of oligosaccharide side chains by weight. Downregulation of this gene has been observed in patients with Crohn disease and ulcerative colitis.

实验方案

Product Specific Protocols
IF protocol for MUC2 antibody 82792-2-RRDownload protocol
IHC protocol for MUC2 antibody 82792-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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