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验证数据展示

  • WB analysis of HeLa using 20077-1-AP (same clone as 20077-1-PBS)

    WB analysis of HeLa using 20077-1-AP (same clone as 20077-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 20077-1-AP (MUC16,CA125 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20077-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 20077-1-AP (same clone as 20077-1-PBS)

    IP experiment of HeLa using 20077-1-AP (same clone as 20077-1-PBS)

    IP result of anti-MUC16,CA125 (IP:20077-1-AP, 4ug; Detection:20077-1-AP 1:600) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 20077-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 20077-1-AP (same clone as 20077-1-PBS)

    IHC staining of human ovary tumor using 20077-1-AP (same clone as 20077-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20077-1-AP (MUC16,CA125 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20077-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 20077-1-AP (same clone as 20077-1-PBS)

    IHC staining of human ovary tumor using 20077-1-AP (same clone as 20077-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20077-1-AP (MUC16,CA125 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20077-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

MUC16/CA125 Polyclonal antibody, PBS Only

MUC16/CA125 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 20077-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, Indirect ELISA

别名

MUC16, MUC16,CA125, MUC-16, MUC 16, CA125

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

20077-1-PBS targets MUC16/CA125 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mucin 16, cell surface associated
别名 MUC16, MUC16,CA125, MUC-16, MUC 16, CA125
计算分子量 1519 kDa
观测分子量 90-100 kDa
GenBank蛋白编号NM_024690
基因名称 MUC16
Gene ID (NCBI) 94025
RRIDAB_10695048
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WXI7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MUC16, also named as CA125, is thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. It is a serum marker that is used routinely in gynecologic practice to monitor patients with ovarian cancer. Most biochemical studies have concluded that MUC16 is a high molecular mass glycoprotein, although estimates of its size range from 200 to 2000 kDa with smaller "subunits" being described by some investigators. The antibody recognizes the C-term of MUC16.

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