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验证数据展示

  • IHC staining of human lung cancer using 13995-1-AP (same clone as 13995-1-PBS)

    IHC staining of human lung cancer using 13995-1-AP (same clone as 13995-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13995-1-AP (MTSS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13995-1-PBS in a different storage buffer formulation.

  • WB analysis using 13995-1-AP (same clone as 13995-1-PBS)

    WB analysis using 13995-1-AP (same clone as 13995-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13995-1-AP (MTSS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13995-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 13995-1-AP (same clone as 13995-1-PBS)

    IF Staining of MCF-7 using 13995-1-AP (same clone as 13995-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using MTSS1 antibody (13995-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13995-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

MTSS1 Polyclonal antibody, PBS Only

MTSS1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 13995-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

13995-1-PBS targets MTSS1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5080

Product name: Recombinant human MTSS1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 126-473 aa of BC023998

Sequence: SQDAFQSKSPSPMPPEAPNQRRKEKREPDPNGGGPTTASGPPAAAEEAQRPRSMTVSAATRPGEEMEACEELALALSRGLQLDTQRSSRDSLQCSSGYSTQTTTPCCSEDTIPSQVSDYDYFSVSGDQEADQQEFDKSSTIPRNSDISQSYRRMFQAKRPASTAGLPTTLGPAMVTPGVATIRRTPSTKPSVRRGTIGAGPIPIKTPVIPVKTPTVPDLPGVLPAPPDGPEERGEHSPESPSVGEGPQGVTSMPSSMWSGQASVNPPLPGPKPSIPEEHRQAIPESEAEDQEREPPSATVSPGQIPESDPADLSPRDTPQGEDMLNAIRRGVKLKKTTTNDRSAPRFS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 metastasis suppressor 1
别名
计算分子量 82 kDa
观测分子量 82 kDa
GenBank蛋白编号BC023998
基因名称 MTSS1
Gene ID (NCBI) 9788
RRIDAB_3085428
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43312
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MTSS1 (metastasis suppressor 1), also known as MIM (missing-in-metastasis), MIM-B, BEG4 (Basal cell carcinoma-enriched gene 4) or KIAA0429, is one of the defining members of the I-BAR family of negative membrane curvature-sensing proteins first identified as being deleted in metastatic bladder cancer. MTSS1 over-expression is associated with enhanced cell migration, resulting in tumorigenesis, invasion and metastasis (PMID: 27230279). It is reported that the expression of MTSS1 is associated with poor prognosis in various cancers,such as bladder, gastric, breast and so on (PMID: 32052679).

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